![Flow Cytometry - CD8 alpha antibody [53-6.7] (PE/Cy5®) (ab95588) image](#CD8-alpha-Primary-antibodies-ab95588-3.jpg)
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Product name
Anti-CD8 alpha antibody [53-6.7] (PE/Cy5®)
See all CD8 alpha products (48) ...
Description
Rat monoclonal [53-6.7] to CD8 alpha (PE/Cy5®)
Conjugation
PE/Cy5®
Tested applications
Flow Cytmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Spleen cells of Mouse origin
Positive control
BALB/c splenocytes; mouse thymocyte and splenocyte suspensions
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark. Do not freeze.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS, 150mM Sodium chloride, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
53-6.7
Isotype
IgG2a
Light chain type
kappa
Research areas
Immunology >> Adaptive Immunity >> T Cells >> CD
Immunology >> Adaptive Immunity >> T Cells >> Cytotoxic Cells
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Flow Cytometry - CD8 alpha antibody [53-6.7] (PE/Cy5®) (ab95588)
![Flow Cytometry - CD8 alpha antibody [53-6.7] (PE/Cy5®) (ab95588) image](/ps/datasheet/images/95/ab95588/CD8-alpha-Primary-antibodies-ab95588-3.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab95588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 0.125µg for 105-8 cells. Staining the cell sample in a final volume of 100 µL is recommended.
Target
Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Post-translational
modifications
All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionP01732
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD8 antibody
- CD8 antigen alpha polypeptide antibody
- CD8 antigen alpha polypeptide (p32) antibody
see all
Database links
- Entrez Gene: 12525 Mouse
- SwissProt: P01731 Mouse
- Unigene: 1858 Mouse
Anti-CD8 alpha antibody [53-6.7] (PE/Cy5®) images:
Flow Cytometry - CD8 alpha antibody [53-6.7] (PE/Cy5®) (ab95588)
Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.06 µg of ab95588 (filled histogram). Total cells were used for analysis.
References for Anti-CD8 alpha antibody [53-6.7] (PE/Cy5®) (ab95588)
This product has been referenced in:
- Yang Z et al. Myocardial infarct-sparing effect of adenosine A2A receptor activation is due to its action on CD4+ T lymphocytes. Circulation 114:2056-64 (2006).Read more (PubMed: 17060376) »
- Taylor JL et al. Factors associated with severe granulomatous pneumonia in Mycobacterium tuberculosis-infected mice vaccinated therapeutically with hsp65 DNA. Infect Immun 73:5189-93 (2005).Read more (PubMed: 16041037) »
See all 3 publications for this product
Publishing research using ab95588? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"