Anti-CD8 alpha antibody [CA9.JD3] (ab34105)
Key features and details
- Mouse monoclonal [CA9.JD3] to CD8 alpha
- Suitable for: IHC-Fr, IP, Flow Cyt
- Reacts with: Dog
- Isotype: IgG2a
Overview
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Product name
Anti-CD8 alpha antibody [CA9.JD3]
See all CD8 alpha primary antibodies -
Description
Mouse monoclonal [CA9.JD3] to CD8 alpha -
Host species
Mouse -
Specificity
Recognizes canine homologue CD8 alpha chain which is expressed by a thymocytes, peripheral T cells and a splenic zone macrophages. -
Tested applications
Suitable for: IHC-Fr, IP, Flow Cytmore details -
Species reactivity
Reacts with: Dog -
Immunogen
Tissue, cells or virus corresponding to Dog CD8 alpha. Dog thymocytes
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Positive control
- Spleen, lymphnode
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General notes
Clone CA9.JD3 has been reported to inhibit cytotoxic T lymphocyte function. Removal of sodium azide is recommended prior to use in functional assays.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Primary antibody notes
Clone CA9.JD3 has been reported to inhibit cytotoxic T lymphocyte function. Removal of sodium azide is recommended prior to use in functional assays. -
Clonality
Monoclonal -
Clone number
CA9.JD3 -
Myeloma
P3x63-Ag8.653 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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|
Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab34105 has been referenced in 2 publications.
- Cobbold S & Metcalfe S Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW). Tissue Antigens 43:137-54 (1994). PubMed: 8091414
- Moore PF et al. Monoclonal antibodies specific for canine CD4 and CD8 define functional T-lymphocyte subsets and high-density expression of CD4 by canine neutrophils. Tissue Antigens 40:75-85 (1992). PubMed: 1412420