Overview

  • Product nameAnti-CD8 alpha antibody [OX-8]See all CD8 alpha primary antibodies ...
  • Description
    Mouse monoclonal [OX-8] to CD8 alpha
  • Tested applicationsBlocking, Flow Cyt, IHC-Fr, IHC-P, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat thymocyte membrane glycoproteins.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Monoclonal
  • Clone numberOX-8
  • MyelomaNS1
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab33786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking Use at an assay dependent dilution.
Flow Cyt 1/50 - 1/100. Use 10µl of the suggested working dilution to label 106 cells in 100µl.
IHC-Fr Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.
WB Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha polypeptide (p32) antibody
    • CD 8a antibody
    • CD8 alpha chain antibody
    • CD8 antibody
    • CD8 antigen alpha chain antibody
    • CD8 antigen alpha polypeptide antibody
    • CD8 antigen alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8a antigen antibody
    • CD8a molecule antibody
    • CD8A_HUMAN antibody
    • Leu 2 antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • Ly3 antibody
    • LYT3 antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • OTTHUMP00000160760 antibody
    • OTTHUMP00000160764 antibody
    • OTTHUMP00000203528 antibody
    • OTTHUMP00000203721 antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T cell coreceptor antibody
    • T cell surface glycoprotein CD8 alpha chain antibody
    • T lymphocyte differentiation antigen T8/Leu 2 antibody
    • T lymphocyte differentiation antigen T8/Leu2 antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    • T8/Leu-2 T-lymphocyte differentiation antigen antibody
    see all

Anti-CD8 alpha antibody [OX-8] images

  • ab33786 staining CD8 alpha in rat spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed with 10% neutral buffered formalin and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then permeabilized with Triton X-100, blocked using 5% serum for 30 minutes at 25°C and then incubated with ab33786 at a 1/100 dilution for 1 hour at 25°C. The secondary used was a biotin-conjugated rabbit anti-mouse polyclonal, used at a 1/300 dilution.

    See Abreview

  • ab33786 staining CD8 alpha in rat spleen tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in acetone, blocked with 5% serum for 30 minutes at 25°C, then incubated with ab33786 at a 1/200 for 1 hour 30 minutes at 25°C. The secondary used was a biotin conjugated rabbit anti-mouse polyclonal, used at a 1/300.

    See Abreview

References for Anti-CD8 alpha antibody [OX-8] (ab33786)

This product has been referenced in:
  • Whiteland JL  et al. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem 43:313-20 (1995). Read more (PubMed: 7868861) »

See 1 Publication for this product

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Thank you for your inquiry.

I am sorry to confirm that we did not map the epitope this antibody binds to and therefore do not know what the homology of epitope with mouse is.

The homology of the whole protein between mouse and rat i...

Read More
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (spleen)
Specification spleen
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Sep 07 2011

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (spleen)
Specification spleen
Fixative 10 % Neutral Buffered Formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization Yes - Triton X-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"