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Overview

  • Product nameCD80 Mouse ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Other biological fluids, Tissue Extracts
  • Assay typeSandwich
  • Sensitivity
    < 10 pg/ml
  • Range
    62.5 pg/ml - 4000 pg/ml
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam's CD80 Mouse ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse CD80 specific-specific polyclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD80 amount of sample captured in plate.

     

    No detectable cross-reactivity with any other cytokine.

  • Notes

    Store at 2-8°C for frequent use, at -20°C for infrequent use.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionInvolved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
  • Tissue specificityExpressed on activated B-cells, macrophages and dendritic cells.
  • Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P33681 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Activation B7-1 antigenB7BB1
        CD80CD80_HUMANCTLA-4 counter-receptor B7.1T-lymphocyte activation antigen CD80
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119578 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      CD80 Mouse ELISA Kit images

      • Sandwich ELISA - CD80 Mouse ELISA Kit (ab119578)
        Sandwich ELISA - CD80 Mouse ELISA Kit (ab119578)
        Representative standard curve using ab119578.

      Protocols

      References for CD80 Mouse ELISA Kit (ab119578)

      ab119578 has not yet been referenced specifically in any publications.

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