CD80 Mouse ELISA Kit (ab119578)
- Product nameCD80 Mouse ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Other biological fluids, Tissue Extracts
- Assay typeSandwich
- Sensitivity< 10 pg/ml
- Range62.5 pg/ml - 4000 pg/ml
- Species reactivityReacts with: Mouse
- Product overview
Abcam's CD80 Mouse ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse CD80 specific-specific polyclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD80 amount of sample captured in plate.
No detectable cross-reactivity with any other cytokine.
Store at 2-8°C for frequent use, at -20°C for infrequent use.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 96-well plate precoated with anti-mouse CD80 antibody 1 unit ABC diluent buffer 1 x 12ml Antibody diluent buffer 1 x 12ml Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti-mouse CD80 antibody 1 x 130µl Lyophilized recombinant mouse CD80 standard 2 vials Sample diluent buffer 1 x 30ml TMB color developing agent 1 x 10ml TMB stop solution 1 x 10ml
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
- Activation B7-1 antigenB7BB1
- CD80CD80_HUMANCTLA-4 counter-receptor B7.1T-lymphocyte activation antigen CD80
Our Abpromise guarantee covers the use of ab119578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for CD80 Mouse ELISA Kit (ab119578)
ab119578 has not yet been referenced specifically in any publications.