PE Anti-CD81 antibody [Eat 2] (ab23553)
Key features and details
- PE Armenian hamster monoclonal [Eat 2] to CD81
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG
Overview
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Product name
PE Anti-CD81 antibody [Eat 2]
See all CD81 primary antibodies -
Description
PE Armenian hamster monoclonal [Eat 2] to CD81 -
Host species
Armenian hamster -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
ab23553 recognises CD81, a 26kD cell surface glycoprotein that is also known as TAPA-1. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: Mouse blood
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General notes
Myeloma: Spleen cells from immunised Armenian hamsters were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line.Purified IgG conjugated to R. Phycoerythrin (RPE).
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
Eat 2 -
Isotype
IgG -
Research areas
Associated products
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Notes |
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Use 10µl for 106 cells in 100µl.
Note: ab23553 induces homotypic aggregation of B cells and inhibits anti Ig and IL4 induced proliferation. ab23553 requires the presence of both extracellular loops of TAPA1 for binding.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV. -
Tissue specificity
Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines. -
Involvement in disease
Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6) [MIM:613496]; also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Post-translational
modificationsNot glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12520 Mouse
- SwissProt: P35762 Mouse
- Unigene: 806 Mouse
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Alternative names
- 26 kDa cell surface protein TAPA 1 antibody
- 26 kDa cell surface protein TAPA-1 antibody
- 26 kDa cell surface protein TAPA1 antibody
see all
Images
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Flow cytometric analysis of mouse blood labelling CD81 with ab23553 at 1/10 dilution (Right). Compared with Alexa Fluor® 700 conjugated hamster IgG1 isotype control (Left). Gated on live, single lymphocytes in the presence of 10% mouse serum. Cells were stained with Alexa Fluor 700 conjugated Rat anti Mouse CD19. Data acquired on the ZE5™ Cell Analyzer.
Datasheets and documents
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Datasheet download
References (0)
ab23553 has not yet been referenced specifically in any publications.