• Product nameAnti-CD82 antibody [5B5]
    See all CD82 primary antibodies
  • Description
    Mouse monoclonal [5B5] to CD82
  • Tested applicationsIHC-P, IHCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Prokaryotic recombinant fusion protein corresponding to the largest of the external domains of the human CD82 molecule.

  • Positive control
    • Tonsil tissue.



Our Abpromise guarantee covers the use of ab49292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/40 - 1/80. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.
IHC Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for IHC-Fr or WB.
  • Target

    • FunctionAssociates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
    • Tissue specificityLymphoid specific.
    • Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4F9 antibody
      • Antigen detected by monoclonal and antibody IA4 antibody
      • C33 antibody
      • C33 antigen antibody
      • CD 82 antibody
      • CD82 antibody
      • Cd82 antibody
      • CD82 antigen antibody
      • CD82 molecule antibody
      • CD82_HUMAN antibody
      • GR 15 antibody
      • GR15 antibody
      • IA 4 antibody
      • IA4 antibody
      • Inducible membrane protein antibody
      • Inducible membrane protein R2 antibody
      • KAI 1 antibody
      • KAI1 antibody
      • Kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4)) antibody
      • Kangai 1 antibody
      • Kangai1 antibody
      • Leukocyte surface antigen R2 antibody
      • Metastasis suppressor Kangai 1 antibody
      • Metastasis suppressor Kangai-1 antibody
      • Metastasis suppressor Kangai1 antibody
      • Prostate cancer antimetastasis gene KAI1 antibody
      • R2 antibody
      • R2 leukocyte antigen antibody
      • SAR 2 antibody
      • SAR2 antibody
      • ST 6 antibody
      • ST6 antibody
      • Suppression of tumorigenicity 6 antibody
      • Suppression of tumorigenicity 6 prostate antibody
      • Suppressor of tumorigenicity 6 antibody
      • Suppressor of tumorigenicity 6 protein antibody
      • Tetraspanin 27 antibody
      • Tetraspanin-27 antibody
      • Tetraspanin27 antibody
      • Tspan 27 antibody
      • Tspan-27 antibody
      • Tspan27 antibody
      see all

    References for Anti-CD82 antibody [5B5] (ab49292)

    This product has been referenced in:
    • Watson SL  et al. The effect of mesenchymal stem cell conditioned media on corneal stromal fibroblast wound healing activities. Br J Ophthalmol 94:1067-73 (2010). Read more (PubMed: 19965828) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (tonsil)
    Specification tonsil
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
    Permeabilization No
    Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 1.5%

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    Submitted Feb 05 2010