Anti-CD82 antibody [B-L2] (Phycoerythrin) (ab27338)

Overview

  • Product nameAnti-CD82 antibody [B-L2] (Phycoerythrin)
    See all CD82 primary antibodies
  • Description
    Mouse monoclonal [B-L2] to CD82 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • SpecificityThis antibody is specific for CD82.
  • Tested applicationsFlow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    CD82 transfected P815 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab27338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells. or 100 µl of whole blood.

Target

  • FunctionAssociates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
  • Tissue specificityLymphoid specific.
  • Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4F9 antibody
    • Antigen detected by monoclonal and antibody IA4 antibody
    • C33 antibody
    • C33 antigen antibody
    • CD 82 antibody
    • CD82 antibody
    • Cd82 antibody
    • CD82 antigen antibody
    • CD82 molecule antibody
    • CD82_HUMAN antibody
    • GR 15 antibody
    • GR15 antibody
    • IA 4 antibody
    • IA4 antibody
    • Inducible membrane protein antibody
    • Inducible membrane protein R2 antibody
    • KAI 1 antibody
    • KAI1 antibody
    • Kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4)) antibody
    • Kangai 1 antibody
    • Kangai1 antibody
    • Leukocyte surface antigen R2 antibody
    • Metastasis suppressor Kangai 1 antibody
    • Metastasis suppressor Kangai-1 antibody
    • Metastasis suppressor Kangai1 antibody
    • Prostate cancer antimetastasis gene KAI1 antibody
    • R2 antibody
    • R2 leukocyte antigen antibody
    • SAR 2 antibody
    • SAR2 antibody
    • ST 6 antibody
    • ST6 antibody
    • Suppression of tumorigenicity 6 antibody
    • Suppression of tumorigenicity 6 prostate antibody
    • Suppressor of tumorigenicity 6 antibody
    • Suppressor of tumorigenicity 6 protein antibody
    • Tetraspanin 27 antibody
    • Tetraspanin-27 antibody
    • Tetraspanin27 antibody
    • Tspan 27 antibody
    • Tspan-27 antibody
    • Tspan27 antibody
    see all

Anti-CD82 antibody [B-L2] (Phycoerythrin) images

  • Ab27338, staining CD82 by FACS.
  • Flow cytometry of HeLa cells treated with trypsin and gated to remove cell debris. Ab27338 was diluted 1/10 and incubated with the cells for 30 minutes at 4°C.

    See Abreview

References for Anti-CD82 antibody [B-L2] (Phycoerythrin) (ab27338)

ab27338 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (HeLa cells)
Specification HeLa cells
Preparation Cell harvesting/tissue preparation method: Cells were collected after PBS-EDTA at 37°C 5min and centrifugate 10 min at 1000 rpm
Sample buffer: Cells were incubated in PBS-1% BSA
Fixation Formaldehyde
Permeabilization No
Username

Dr. Lydia Danglot

Verified customer

Submitted Nov 13 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Hela)
Specification Hela
Preparation Cell harvesting/tissue preparation method: trypsin
Sample buffer: PBS 1X
Fixation no fixation
Permeabilization No
Gating Strategy without debris
Username

Abcam user community

Verified customer

Submitted Nov 06 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"