Anti-CD82 antibody [TS82b] - Azide free (ab59509)

Overview

• Product nameAnti-CD82 antibody [TS82b] - Azide freeSee all CD82 primary antibodies ...
• Description
  Mouse monoclonal [TS82b] to CD82 - Azide free
• Tested applicationsWB, IP, Flow Cyt more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Jurkat and HEL cell lines.

• Positive controlHEL cells

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferCarrier and preservative free
  Constituents: PBS
• Concentration information loading...
• PurityIon Exchange Chromatography
• Purification notesSterile-filtered through 0.22 µm.
• Clonality Monoclonal
• Clone numberTS82b
• MyelomaP3-x63-Ag8
• IsotypeIgG1
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • Other

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Cancer susceptibility
  • Tumor Suppressors

  • Immunology
  • Cell Type Markers
  • CD
  • Platelets

  • Immunology
  • Adaptive Immunity
  • T Cells
  • CD

  • Immunology
  • Cell Type Markers
  • CD
  • NK Cells

Applications

Target

• FunctionAssociates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
• Tissue specificityLymphoid specific.
• Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
• Cellular localizationMembrane.

Target information above from: UniProt accession P27701 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3732 Human
  • Omim: 600623 Human
  • SwissProt: P27701 Human
  • Unigene: 527778 Human

• Alternative names
  4F9 antibodyAntigen detected by monoclonal and antibody IA4 antibodyC33 antibody

  C33 antigen antibodyCD 82 antibodyCD82 antibodyCd82 antibodyCD82 antigen antibodyCD82 molecule antibodyCD82_HUMAN antibodyGR 15 antibodyGR15 antibodyIA 4 antibodyIA4 antibodyInducible membrane protein antibodyInducible membrane protein R2 antibodyKAI 1 antibodyKAI1 antibodyKangai 1 antibodyKangai1 antibodyMetastasis suppressor Kangai 1 antibodyMetastasis suppressor Kangai-1 antibodyMetastasis suppressor Kangai1 antibodyR2 antibodyR2 leukocyte antigen antibodySAR 2 antibodySAR2 antibodyST 6 antibodyST6 antibodySuppression of tumorigenicity 6 antibodySuppression of tumorigenicity 6 prostate antibodySuppressor of tumorigenicity 6 antibodySuppressor of tumorigenicity 6 protein antibodyTetraspanin 27 antibodyTetraspanin-27 antibodyTetraspanin27 antibodyTspan 27 antibodyTspan-27 antibodyTspan27 antibody

  see all

Anti-CD82 antibody [TS82b] - Azide free images

• 

  Western blot - Anti-CD82 antibody [TS82b] - Azide free (ab59509)
  
  
  Predicted band size : 37 kDa
  
  
  1,3 10⁵ HEL cells were lysed (lysis buffer containing 1%Brij97 or triton) Migration SDS/page 5-15% gel under non-reducing conditions.
• 

  Flow Cytometry - Anti-CD82 antibody [TS82b] - Azide free (ab59509)
  Overlay histogram showing HeLa cells stained with ab59509 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59509, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
  Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.