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- Secondary antibodies
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Product name
Anti-CD82 antibody
See all CD82 products (9) ...
Description
Rabbit polyclonal to CD82
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A 15 amino acid peptide from near the carboxy terminus of human CD82
Positive control
A549 cell lysate
Properties
Form
Liquid
Storage instructions
Store at 4°C for up to one year. Upon use, aliquot and store at -20°C or -80°C.
Storage buffer
Preservative: 0.2% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purification notes
Purified IgG
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Immunology >> Cell Type Markers >> CD >> Platelets
Immunology >> Cell Type Markers >> CD >> NK Cells
Immunology >> Adaptive Immunity >> T Cells >> CD
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 30 kDa.
IHC-P: Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Target
Function
Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
Tissue specificity
Lymphoid specific.
Sequence similarities
Belongs to the tetraspanin (TM4SF) family.
Cellular localization
Membrane.
Target information above from: UniProt accessionP27701
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 4F9 antibody
- Antigen detected by monoclonal and antibody IA4 antibody
- C33 antibody
see all
Database links
- Entrez Gene: 3732 Human
- Entrez Gene: 12521 Mouse
- Entrez Gene: 83628 Rat
- Omim: 600623 Human
- SwissProt: Q7Z2D4 Human
- SwissProt: P27701 Human
- SwissProt: P40237 Mouse
- SwissProt: O70352 Rat
see all
Anti-CD82 antibody images:
Western blot - CD82 antibody (ab58830)
Lane 1 : Anti-CD82 antibody (ab58830) at 0.5 µg/ml
Lane 2 : Anti-CD82 antibody (ab58830) at 1 µg/ml
Lane 1 : A549 cell lysate
Lane 2 : A549 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 30 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CD82 antibody(ab58830)
IHC image of ab58830 staining in human cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58830, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-CD82 antibody (ab58830)
ab58830 has not yet been referenced specifically in any publications.
Publishing research using ab58830? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"