Overview
- Product nameAnti-CD82 antibodySee all CD82 primary antibodies ...
- DescriptionRabbit polyclonal to CD82
- Tested applicationsWB, IHC-P, ICC/IF more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human CD82.
(Peptide available as ab71584.)
- Positive controlThis antibody gave a positive signal in Brain (Human) Tissue Lysate
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab66400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 30 kDa).Can be blocked with CD82 peptide (ab71584). |
| IHC-P | IHC-P: 1/50 - 1/80. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
| ICC/IF | ICC/IF: Use at an assay dependent concentration. PubMed: 22679510 |
Target
- FunctionAssociates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
- Tissue specificityLymphoid specific.
- Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
- Cellular localizationMembrane.
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Database links
- Entrez Gene: 3732 Human
- Entrez Gene: 12521 Mouse
- Entrez Gene: 83628 Rat
- Omim: 600623 Human
- SwissProt: P27701 Human
- SwissProt: P40237 Mouse
- SwissProt: O70352 Rat
- Unigene: 527778 Human
- Unigene: 4261 Mouse
- Unigene: 3022 Rat
see all
Target information above from: UniProt accession
P27701
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 4F9 antibodyAntigen detected by monoclonal and antibody IA4 antibodyC33 antibody
- C33 antigen antibodyCD 82 antibodyCD82 antibodyCd82 antibodyCD82 antigen antibodyCD82 molecule antibodyCD82_HUMAN antibodyGR 15 antibodyGR15 antibodyIA 4 antibodyIA4 antibodyInducible membrane protein antibodyInducible membrane protein R2 antibodyKAI 1 antibodyKAI1 antibodyKangai 1 antibodyKangai1 antibodyMetastasis suppressor Kangai 1 antibodyMetastasis suppressor Kangai-1 antibodyMetastasis suppressor Kangai1 antibodyR2 antibodyR2 leukocyte antigen antibodySAR 2 antibodySAR2 antibodyST 6 antibodyST6 antibodySuppression of tumorigenicity 6 antibodySuppression of tumorigenicity 6 prostate antibodySuppressor of tumorigenicity 6 antibodySuppressor of tumorigenicity 6 protein antibodyTetraspanin 27 antibodyTetraspanin-27 antibodyTetraspanin27 antibodyTspan 27 antibodyTspan-27 antibodyTspan27 antibody
see all
Anti-CD82 antibody images
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Western blot - CD82 antibody (ab66400)Anti-CD82 antibody (ab66400) at 1 µg/ml +Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 30 kDa
Observed band size : 34 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD82 antibody (ab66400)ab66400 (1:80) staining CD82 in paraffin-embedded human tonsil tissue (left panel) using an automated system (Ventana Discovery). Right hand panel shows negative control (no primary antibody).
Using this protocol there is strong membrane staining of follicular dendritic cells, and the FDC network forming the structure of germinal centres is clearly highlighted by this antibody. There is associated weak staining of smaller cells in the interfollicar areas which may be T cells.Sections were rehydrated and antigen retrieved in CC1 Cell Conditioning Buffer using Ventana Standard Retrieval programme. Slides were blocked in 3% H2O2 / 4 min / 37°C and incubated with ab66400 (1:80 dilution / 1 hour / 37°C). Sections then blocked (4mins / 37°C) and incubated with Dako swine anti-rabbit antibody (1:50, 28 min / 37°C). Staining was amplified and detected by incubation with Ventana Streptavidin ABC system (16 min / 37°C) and Ventana DAB map reagent (8 min / 37°C). Slides were counterstained with Haematoxylin and coverslipped in DPX.
For manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD82 antibody (ab66400)This image is courtesy of an abreview submitted by Antibody Solutions Ltd.ab66400 (1/50) staining CD82 in paraffin-embedded Mouse salivary gland tissue. Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. The secondary antibody was goat anti rabbit/mouse conjugated to HRP. For further experimental details please refer to abreview.
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Immunocytochemistry/ Immunofluorescence - Anti-CD82 antibody (ab66400)Image from Zhang Q et al., PLoS One. 2012;7(6):e38487. Epub 2012 Jun 5. Fig 1.; doi:10.1371/journal.pone.0038487; June 5, 2012, PLoS ONE 7(6): e38487.Immunofluorescence analysis of HTR8/SVneo cell lines, staining CD82 (green) with ab66400. A FITC-conjugated anti-rabbit IgG (1/100) was used as the secondary antibody. Nuclei were detected by DAPI staining (blue).
References for Anti-CD82 antibody (ab66400)
This product has been referenced in:
- Zhang Q et al. Expression of CD82 in human trophoblast and its role in trophoblast invasion. PLoS One 7:e38487 (2012). WB, ICC/IF ; Human . Read more (PubMed: 22679510) »
