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Overview

  • Product nameCD86 Human ELISA KitSee all CD86 kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sample A 8 37.25U/ml 0.329 0.88%
    Sample B 8 5.09U/ml 0.193 3.79%
    Inter-assay
    Sample n Mean SD CV%
    Sample A 39 37.63U/ml 0.611 1.62%
    Sample B 39 4.92U/ml 0.348 7.06%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    = 0.6 U/ml
  • Range
    1.187 U/ml - 38 U/ml
  • Assay time
    2h 40m
  • Species reactivity
    Reacts with: Human
  • Product overview

    The Human CD86 ELISA is to be used for the in-vitro quantitative determination of soluble CD86 (CD86) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human CD86. This kit has been configured for research use only and is not to be used in diagnostic procedures.

    Principal:

    The CD86 Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for CD86 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD86 concentrations and unknowns are pipetted into these wells. During the first incubation, the CD86 antigen and a biotinylated monoclonal antibody specific for CD86 are simultaneously incubated. After washing, the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD86 present in the samples.

     

    Recognizes natural human CD86

    Range: 1.187 U/ml - 38 U/ml

    Sensitivity <0.7 U/ml

  • Notes

    Safety Information:

    • For research use only.

    • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.

    • Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly with water.

    • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.

    • Do not pipette by mouth.

    • The stop solution in this kit contains 9.8% sulphuric acid.

  • Tested applicationsELISA more details

Properties

  • RelevanceCD86 is a member of the immunoglobulin superfamily. It is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined.
  • Cellular localizationCell Membrane; single-pass type I membrane protein.
    • Alternative names
        Activation B7 2 antigenB7 2B70
        B72BU63 CD28LG2 CD86 antigen (CD28 antigen ligand 2 B7 2 antigen) CD86 moleculeCTLA 4 counter receptor B7.2 FUN 1 FUN1LAB72MGC34413T lymphocyte activation antigen CD86 precursor
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab45920 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      CD86 Human ELISA Kit images

      • ELISA - Human CD86 ELISA Kit (ab45920)
        ELISA - Human CD86 ELISA Kit (ab45920)
        Typical CD86 standard curve ranging from 1.19 to 38 U/ml.

      References for CD86 Human ELISA Kit (ab45920)

      ab45920 has not yet been referenced specifically in any publications.

      Publishing research using ab45920 ? Please let us know so that we can cite the reference in this datasheet

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      I am looking to use the kit on protein lysates from human cancer cells. It is not quite clear from the product details if the kit has ever been used in this application. Please can you qualify if the kit has ever been used on protein lysates from cell lin...

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      We have not tested thisCD86 ELISA kit with cell lysates and therefore have no data to share. However, the lysis protocol mentioned is commonly used in other ELISAs with good results (I mean there is no interference) so this is highly likely it w...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"