FITC Anti-CD86 antibody [BU63] (ab77276)
Key features and details
- FITC Mouse monoclonal [BU63] to CD86
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
FITC Anti-CD86 antibody [BU63]
See all CD86 primary antibodies -
Description
FITC Mouse monoclonal [BU63] to CD86 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD86. B-lymphoblastoid cell line ARH 77
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Positive control
- Human blood cells.
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General notes
The antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituent: 0.2% BSA
0.2% (w/v) high-grade protease free BSA -
Concentration information loading...
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Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
BU63 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- APC Anti-CD86 antibody [BU63], prediluted (ab134385)
- Anti-CD86 antibody [BU63] - BSA and Azide free (ab213044)
- PE/Cy7® Anti-CD86 antibody [BU63] (ab233571)
- Anti-CD86 antibody [BU63] (ab234000)
- PE/Cy5® Anti-CD86 antibody [BU63] (ab234226)
- PerCP Anti-CD86 antibody [BU63] (ab77131)
- PE Anti-CD86 antibody [BU63] (ab77226)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
Use 20 μl per 100 μl of whole blood or 106 cells |
Notes |
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Flow Cyt
Use at an assay dependent concentration. Use 20 μl per 100 μl of whole blood or 106 cells |
Target
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Function
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. -
Tissue specificity
Expressed by activated B-lymphocytes and monocytes. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPolyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 942 Human
- Omim: 601020 Human
- SwissProt: P42081 Human
- Unigene: 171182 Human
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Alternative names
- Activation B7-2 antigen 3 antibody
- Activation B7-2 antigen antibody
- B-lymphocyte activation antigen B7-2 2 antibody
see all
Images
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Separation of human monocytes (red-filled) from CD86 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab77276 (20 μl reagent / 100 μl of peripheral whole blood).
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Flow cytometry surface staining pattern of human peripheral whole blood stained using ab77276 (20 μl reagent / 100 μl of peripheral whole blood).
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab77276 has been referenced in 8 publications.
- Wang Q et al. Cichoric Acid Ameliorates Monosodium Urate-Induced Inflammatory Response by Reducing NLRP3 Inflammasome Activation via Inhibition of NF-kB Signaling Pathway. Evid Based Complement Alternat Med 2021:8868527 (2021). PubMed: 33505510
- Shrestha P et al. Pomalidomide restores immune recognition of primary effusion lymphoma through upregulation of ICAM-1 and B7-2. PLoS Pathog 17:e1009091 (2021). PubMed: 33411730
- Liu S et al. Iguratimod promotes transformation of mononuclear macrophages in elderly patients with rheumatoid arthritis by nuclear factor-?B pathway. World J Clin Cases 9:2181-2191 (2021). PubMed: 33869594
- Cao X et al. Y-box binding protein 1 regulates ox-LDL mediated inflammatory responses and lipid uptake in macrophages. Free Radic Biol Med 141:10-20 (2019). PubMed: 31153975
- Giguère JF et al. Insertion of host-derived costimulatory molecules CD80 (B7.1) and CD86 (B7.2) into human immunodeficiency virus type 1 affects the virus life cycle. J Virol 78:6222-32 (2004). PubMed: 15163715
- Mauri D et al. Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules. J Immunol 155:118-27 (1995). PubMed: 7541409
- Engel P et al. The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen. Blood 84:1402-7 (1994). PubMed: 7520767
- Caux C et al. B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells. J Exp Med 180:1841-7 (1994). PubMed: 7525840