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Products:Cardiovascular >> Blood >> Platelets
Overview
Product name
Anti-CD9 antibody [MEM-61]
See all CD9 products (29) ...
Description
Mouse monoclonal [MEM-61] to CD9
Specificity
This antibody reacts with human CD9 antigen.
Tested applications
WB, IP, IHC-P, Flow Cyt, Functional Studiesmore details
Cross reactivity
Reacts with
Human
Immunogen
Tissue / cell preparation (Human). (Pre-B line Nalm-6).
Positive control
Prostate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
Purified from ascities by protein-A affinity chromatography.
Clonality
Monoclonal
Clone number
MEM-61
Myeloma
unknown
Isotype
IgG1
Light chain type
unknown
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Intracellular
Developmental Biology >> Reproduction >> Fertilisation
Stem Cells >> Embryonic Stem Cells >> Surface Molecules
Immunology >> Cell Type Markers >> CD >> Platelets
Cardiovascular >> Blood >> Other
Cardiovascular >> Blood >> Platelets
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 2 - 4 µg/ml. Use under non reducing condition.
IP: Use at an assay dependent dilution.
IHC-P: Use a concentration of 20 µg/ml
Flow Cyt: Use a concentration of 5 µg/ml
FuncS: Use at an assay dependent concentration. (The antibody induces FcgR-dependent platelet activation (aggregation).)
Target
Function
Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
Tissue specificity
Expressed by a variety of hematopoietic and epithelial cells.
Sequence similarities
Belongs to the tetraspanin (TM4SF) family.
Post-translational
modifications
Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.
Cellular localization
Membrane.
Target information above from: UniProt accessionP21926
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Tetraspanin 29 antibody
- 5H9 antibody
- 5H9 antigen antibody
see all
Database links
- Entrez Gene: 928 Human
- Omim: 143030 Human
- SwissProt: P21926 Human
- Unigene: 114286 Human
References for Anti-CD9 antibody [MEM-61] (ab2215)
This product has been referenced in:
- Singh ABet al. Juxtacrine activation of EGFR regulates claudin expression and increases transepithelial resistance. Am J Physiol Cell Physiol 293:C1660-8 (2007).Read more (PubMed: 17855771) »
- Singh ABet al. Membrane-associated HB-EGF modulates HGF-induced cellular responses in MDCK cells. J Cell Sci 117:1365-79 (2004).Read more (PubMed: 14996914) »
See all 3 publications for this product
Publishing research using ab2215? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"