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Products:Cardiovascular >> Blood >> Platelets
Overview
Product name
Anti-CD9 antibody
See all CD9 products (29) ...
Description
Rabbit polyclonal to CD9
Specificity
Ab27542 recognises CD9.
Tested applications
IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length CD9 protein (Human).
Positive control
HeLa cells, human tonsil.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Ab27542 is affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Intracellular
Developmental Biology >> Reproduction >> Fertilisation
Stem Cells >> Embryonic Stem Cells >> Surface Molecules
Immunology >> Cell Type Markers >> CD >> Platelets
Cardiovascular >> Blood >> Other
Cardiovascular >> Blood >> Platelets
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Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - CD9 antibody (ab27542)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab27542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IHC-P
Application notes
IHC-P: 1/50 for 10 min at room temperature. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM citrate buffer pH 6.0 for 10 min and then cool at room temperature for 20 min.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
Tissue specificity
Expressed by a variety of hematopoietic and epithelial cells.
Sequence similarities
Belongs to the tetraspanin (TM4SF) family.
Post-translational
modifications
Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.
Cellular localization
Membrane.
Target information above from: UniProt accessionP21926
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Tetraspanin 29 antibody
- 5H9 antibody
- 5H9 antigen antibody
see all
Database links
- Entrez Gene: 928 Human
- Omim: 143030 Human
- SwissProt: P21926 Human
- Unigene: 114286 Human
Anti-CD9 antibody images:
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - CD9 antibody (ab27542)
Ab27542, at a dilution of 1/50, staining CD9 in formalin fixed paraffin embedded human tonsil tissue section with by Immunohistochemistry.
References for Anti-CD9 antibody (ab27542)
ab27542 has not yet been referenced specifically in any publications.
Publishing research using ab27542? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"