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Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - CD9 antibody (ab27542)
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Product Name
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CD9 antibody
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See all CD9 antibodies (22)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to CD9
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Immunogen
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Recombinant full length CD9 protein (Human).
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Reacts with
(species key)
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Hu
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Specificity
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Ab27542 recognises CD9.
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Tested applications
(see key)
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IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/50 for 10 min at room temperature. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM citrate buffer pH 6.0 for 10 min and then cool at room temperature for 20 min.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cells, human tonsil.
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Cellular localization
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Integral membrane protein.
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Research areas
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Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Intracellular
Developmental Biology >> Reproduction >> Fertilisation
Stem Cells >> Embryonic Stem Cells >> Surface Molecules
Immunology >> Cell Type Markers >> CD >> Platelets
Cardiovascular >> Blood >> Other
Cardiovascular >> Blood >> Platelets
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Relevance
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CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). It also regulates cell motility in a variety of cell lines, and appears to be an important regulator of Schwann cell behaviour in peripheral nerve.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Ab27542 is affinity purified.
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD9 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD9 antibody images, references, products related to ab27542 and other tools.
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CD9 antibody images:
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 Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - CD9 antibody (ab27542)
Ab27542, at a dilution of 1/50, staining CD9 in formalin fixed paraffin embedded human tonsil tissue section with by Immunohistochemistry.
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Search PubMed (MEDLINE) for references to CD9
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CD9 antibody - more information
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Customer reviews (feedback) regarding CD9 antibody |
Customer FAQs regarding CD9 antibody |
Protocols for CD9 antibody |
Price and availability of products related to CD9 antibody |
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CD9 antibody - related products:
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CD9 antibody - other tools:
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Contact Abcam with a Technical Enquiry about CD9 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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