![Immunocytochemistry/ Immunofluorescence - CD90 / Thy1 antibody [30-H12] (Biotin) (ab19630) image](#ab19630_1.jpg)
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Products:Immunology >> Adaptive Immunity >> T Cells >> CD
Overview
Product name
Anti-CD90 / Thy1 antibody [30-H12] (Biotin)
See all CD90 / Thy1 products (41) ...
Description
Rat monoclonal [30-H12] to CD90 / Thy1 (Biotin)
Conjugation
Biotin
Specificity
Recognizes the Thy-1.2 leukocyte marker.
Tested applications
ICC/IF, IF, Flow Cyt, WB, IPmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Unfortunately, this information is considered to be commercially sensitive
General notes
This product is conjugated via the primary amines with approximately 4-8 molecules of biotin per IgG.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
30-H12
Isotype
IgG2b
Research areas
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Immunology >> Adaptive Immunity >> T Cells >> CD
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Immunocytochemistry/ Immunofluorescence - CD90 / Thy1 antibody [30-H12] (Biotin) (ab19630)
![Immunocytochemistry/ Immunofluorescence - CD90 / Thy1 antibody [30-H12] (Biotin) (ab19630) image](/ps/datasheet/Images/19/ab19630/ab19630_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab19630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF
Flow Cyt
WB
IP
Application notes
Flow Cyt: Use 1µg for 106 cells.
IF: Use at an assay dependent dilution.
ICC/IF; Use at 1;100
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 18 kDa. Dilution optimised using Chromogenic detection.
Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP04216
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
see all
Database links
- Entrez Gene: 21838 Mouse
- SwissProt: P01831 Mouse
- Unigene: 3951 Mouse
Anti-CD90 / Thy1 antibody [30-H12] (Biotin) images:
Immunocytochemistry/ Immunofluorescence - CD90 / Thy1 antibody [30-H12] (Biotin) (ab19630)
ab19630 at 1/100 staining mouse ES cell derived hepatocyte precursors by ICC/IF. The cells were paraformaldehyde fixed and permeabilized with Triton X-100 before being blocked with BSA and incubated with the antibody for 24 hours at 4°C.
This image is courtesy of an Abreview submitted by Dr Eugen Kolossov
References for Anti-CD90 / Thy1 antibody [30-H12] (Biotin) (ab19630)
ab19630 has not yet been referenced specifically in any publications.
Publishing research using ab19630? Please let us know so that we can cite the reference in this datasheet
![CD90 / Thy1 antibody [30-H12] (Biotin) for ICC/IF in Mouse (19630)](/ps/Reviews/Images/ab8786_42371.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"