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Immunocytochemistry/ Immunofluorescence - CD90 / Thy1 antibody [30-H12] (Biotin) (ab19630)
![Immunocytochemistry/ Immunofluorescence - CD90 / Thy1 antibody [30-H12] (Biotin) (ab19630)](/ps/datasheet/images/ab19630_1.jpg)
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Product Name
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CD90 / Thy1 antibody [30-H12] (Biotin)
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See all CD90 / Thy1 antibodies (23)...
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Product type
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Primary antibodies
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Description
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Rat monoclonal [30-H12] to CD90 / Thy1 (Biotin)
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Immunogen
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Unfortunately, this information is considered to be commercially sensitive
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Reacts with
(species key)
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Ms
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Specificity
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Recognizes the Thy-1.2 leukocyte marker.
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use 1µg for 106 cells.
IF: Use at an assay dependent dilution.
ICC/IF; Use at 1;100
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 18 kDa. Dilution optimised using Chromogenic detection.
Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Attached to the membrane by a GPI anchor.
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Research areas
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Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Immunology >> Adaptive Immunity >> T Cells >> CD
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Relevance
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CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rat
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Clonality
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Monoclonal
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Clone number
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30-H12
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Isotype
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IgG2b
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Conjugation
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Biotin
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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Notes
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This product is conjugated via the primary amines with approximately 4-8 molecules of biotin per IgG.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD90 / Thy1 antibody [30-H12] (Biotin) is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD90 / Thy1 antibody [30-H12] (Biotin) images, references, products related to ab19630 and other tools.
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CD90 / Thy1 antibody [30-H12] (Biotin) images:
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 Immunocytochemistry/ Immunofluorescence - CD90 / Thy1 antibody [30-H12] (Biotin) (ab19630)
ab19630 at 1/100 staining mouse ES cell derived hepatocyte precursors by ICC/IF. The cells were paraformaldehyde fixed and permeabilized with Triton X-100 before being blocked with BSA and incubated with the antibody for 24 hours at 4°C. This image is courtesy of an Abreview submitted by Dr Eugen Kolossov
See Abreview
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Search PubMed (MEDLINE) for references to CD90 / Thy1
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CD90 / Thy1 antibody [30-H12] (Biotin) - more information
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Customer reviews (feedback) regarding CD90 / Thy1 antibody [30-H12] (Biotin) |
Customer FAQs regarding CD90 / Thy1 antibody [30-H12] (Biotin) |
Protocols for CD90 / Thy1 antibody [30-H12] (Biotin) |
Price and availability of products related to CD90 / Thy1 antibody [30-H12] (Biotin) |
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CD90 / Thy1 antibody [30-H12] (Biotin) - related products:
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CD90 / Thy1 antibody [30-H12] (Biotin) - other tools:
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Contact Abcam with a Technical Enquiry about CD90 / Thy1 antibody [30-H12] (Biotin) |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunocytochemistry/ Immunofluorescence - CD90 / Thy1 antibody [30-H12] (Biotin) (ab19630)](/index.html?datasheet=19630#ab19630_1.jpg)
