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Anti-CD90 / Thy1 antibody [MRC OX-7] (ab225)

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Overview

Product name

Anti-CD90 / Thy1 antibody [MRC OX-7]
See all CD90 / Thy1 products (41) ...

Description

Mouse monoclonal [MRC OX-7] to CD90 / Thy1

Specificity

Recognises the Thy-1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in mice. Thus MRC OX-7 reacts with Thy-1.1 mice eg. AKR strain, but not Thy-1.2 mice eg. CBA, BALB/c. As Thy 1 is monomorphic in rat it will react with all rat strains.

Tested applications

Flow Cyt, IP, WB, ICC, IHC-Frmore details

Cross reactivity

Reacts with

Mouse, Rat, Rabbit, Guinea pig

Immunogen

Full length protein (Rat).

General notes

The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 109m-1 and for mouse Thy-1.1 is 3 x 108m-1.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

PBS with 0.1% sodium azide

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified IgG prepared by affinity chromotography on protein G from tissue culture supernatant.

Clonality

Monoclonal

Clone number

MRC OX-7

Myeloma

NS1

Isotype

IgG1

Research areas

Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Immunology >> Adaptive Immunity >> T Cells >> CD

  • Immunohistochemistry - CD90 / Thy1 antibody [MRC OX-7] (ab225)Immunohistochemistry - CD90 / Thy1 antibody [MRC OX-7] (ab225) image (enlarge)

  • Flow Cytometry - CD90 / Thy1 antibody [MRC OX-7] (ab225)Flow Cytometry - CD90 / Thy1 antibody [MRC OX-7] (ab225) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Sequence similarities

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Cellular localization

Cell membrane.

Target information above from: UniProt accessionP04216 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CD7 antibody
  • CD90 antibody
  • CD90 antigen antibody
  • CDw90 antibody
  • FLJ33325 antibody
  • MGC128895 antibody
  • T25 antibody
  • Theta antigen antibody
  • Thy 1 antibody
  • Thy 1 cell surface antigen antibody
  • Thy 1 membrane glycoprotein antibody
  • Thy 1 membrane glycoprotein precursor antibody
  • Thy 1 T cell antigen antibody
  • Thy 1.2 antibody
  • Thy-1 antigen antibody
  • Thy-1 membrane glycoprotein antibody
  • Thy-1 T-cell antigen antibody
  • Thy1 antibody
  • Thy1 antigen antibody
  • Thy1 T cell antigen antibody
  • Thy1.1 antibody
  • Thy1.2 antibody
  • THY1_HUMAN antibody
  • Thymus cell antigen 1, theta antibody
see all

Database links

Anti-CD90 / Thy1 antibody [MRC OX-7] images:

  Immunohistochemistry - CD90 / Thy1 antibody [MRC OX-7] (ab225)

Immunohistochemistry - CD90 / Thy1 antibody [MRC OX-7] (ab225)

This image was kindly supplied as part of the review submitted by Nick Voilley. Rat retinal ganglion cell labelled with ab225 as a primary antibody and an anti-mouse F(ab)' 2 coupled to Alexa488 as a secondary antibody. The cell is approximately 15 micrometers in diameter. Only the cells labelled in green in the culture bear action potentials when stimulated. These three elements (reactivity to ab225, size and elecrophysiological parameters) clearly indicate the cell is a ganglion cell.

  Flow Cytometry - CD90 / Thy1 antibody [MRC OX-7] (ab225)

Flow Cytometry - CD90 / Thy1 antibody [MRC OX-7] (ab225)

ab225 staining CD90 / Thy1 in rat thymus by Flow Cytometry analysis.

References for Anti-CD90 / Thy1 antibody [MRC OX-7] (ab225)

This product has been referenced in:

  • Allodi Iet al. In vitro comparison of motor and sensory neuron outgrowth in a 3D collagen matrix. J Neurosci Methods 198:53-61 (2011).Read more (PubMed: 21402104) »
  • Kueh JLet al. Comparison of bulbar and mucosal olfactory ensheathing cells using FACS and simultaneous antigenic bivariate cell cycle analysis. Glia 59:1658-71 (2011).Read more (PubMed: 21748806) »

See all 6 publications for this product

Publishing research using ab225? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"