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Product Name
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CD95 antibody [5F-7]
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See all CD95 antibodies (37)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [5F-7] to CD95
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Immunogen
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Recombinant full length protein (Human).
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000. IP: Use 2-5µg for 106 cells. WB: 1/1000. Predicted molecular weight: 38 kDa. Dilution optimised using Chromogenic detection. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Single pass type I membrane protein (isoform 1); Secreted (isoforms 2 to 6).
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Research areas
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Cancer >> Signal transduction >> Other
Cancer >> Growth factors >> TNF
Signal Transduction >> Growth Factors/Hormones >> TNF
Signal Transduction >> Adapters >> Transmembrane
Immunology >> Innate Immunity >> Cytokines >> TNF Superfamily
Immunology >> Cell Type Markers >> CD >> Cytokines
Immunology >> Immune Interventions >> Transplantation
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Cell Biology >> Apoptosis >> Receptors >> Death Receptors
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Relevance
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CD95, also known as FAS or APO1, is a 36 kDa cell surface type I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS PAGE. CD95 is a member of the TNF receptor family, which includes TNFR1, TNFR2, CD27, CD30 and CD40. Binding of CD95 Ligand to CD95 or crosslinking of CD95 by anti CD95 monoclonal antibodies leads to apoptosis of CD95 expressing cells. CD95 belongs to a subgroup of family members that have a death domain (DD) which contains 70 amino acids near the carboxyl terminal region of the molecule. The binding of adaptor molecules to this DD is responsible for transmitting the death signal for apoptosis. Stimulation of CD95 results in aggregation of its DD, leading to the recruitment of FADD and caspase 8 that together with the receptor form the death inducing signaling complex (DISC). CD95/CD95L is involved in the peripheral deletion of activated mature T cells at the end of the immune response and defects in this pathway predispose to autoimmune disorders. CD95 is also involved in killing of targets such as virus infected cells or cancer cells and killing of inflammatory cells at immune privileged sites.
ab19454 does not induce apoptosis
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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5F-7
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Isotype
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IgG1
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD95 antibody [5F-7] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to CD95
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CD95 antibody [5F-7] - more information
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Customer reviews (feedback) regarding CD95 antibody [5F-7] |
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