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Products:Cell Biology >> Apoptosis >> Receptors >> Death Receptors
Overview
Product name
Anti-CD95 antibody [5F-7]
See all CD95 products (37) ...
Description
Mouse monoclonal [5F-7] to CD95
Tested applications
IP, WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Primary antibody notes
ab19454 does not induce apoptosis
Clonality
Monoclonal
Clone number
5F-7
Isotype
IgG1
Research areas
Cancer >> Signal transduction >> Other
Cancer >> Growth factors >> TNF
Signal Transduction >> Growth Factors/Hormones >> TNF
Signal Transduction >> Adapters >> Transmembrane
Immunology >> Innate Immunity >> Cytokines >> TNF Superfamily
Immunology >> Cell Type Markers >> CD >> Cytokines
Immunology >> Immune Interventions >> Transplantation
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Cell Biology >> Apoptosis >> Receptors >> Death Receptors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab19454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
WB
ELISA
Application notes
ELISA: 1/1000. IP: Use 2-5µg for 106 cells. WB: 1/1000. Predicted molecular weight: 38 kDa. Dilution optimised using Chromogenic detection. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Tissue specificity
Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
Involvement in disease
Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
Sequence similarities
Contains 1 death domain.
Contains 3 TNFR-Cys repeats.
Domain
Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionP25445
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ALPS 1A antibody
- ALPS1A antibody
- APO 1 antibody
see all
Database links
- Entrez Gene: 355 Human
- Omim: 134637 Human
- SwissProt: P25445 Human
- Unigene: 244139 Human
References for Anti-CD95 antibody [5F-7] (ab19454)
ab19454 has not yet been referenced specifically in any publications.
Publishing research using ab19454? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"