![Western blot - CD95 antibody [IPO-4] (ab19456) image](#CD95-Primary-antibodies-ab19456-2.jpg)
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Products:Cell Biology >> Apoptosis >> Receptors >> Death Receptors
Overview
Product name
Anti-CD95 antibody [IPO-4]
See all CD95 products (37) ...
Description
Mouse monoclonal [IPO-4] to CD95
Tested applications
Functional Studies, Flow Cyt, IHC-Fr, WBmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Tissue / cell preparation (Human).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
IPO-4
Isotype
IgM
Research areas
Immunology >> Innate Immunity >> Cytokines >> TNF Superfamily
Immunology >> Cell Type Markers >> CD >> Cytokines
Cell Biology >> Apoptosis >> Receptors >> Death Receptors
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Western blot - CD95 antibody [IPO-4] (ab19456)
![Western blot - CD95 antibody [IPO-4] (ab19456) image](/ps/datasheet/images/19/ab19456/CD95-Primary-antibodies-ab19456-2.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab19456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS: Use a concentration of 0.2 - 1 µg/ml.
Flow Cyt: Use a concentration of 0.1 - 1.0 µg/ml.
IHC-Fr: Use a concentration of 1.0 - 10.0 µg/ml.
WB: Use a concentration of 5 mg/mlDetects a band of approximately 48 kDa (predicted molecular weight: 45 kDa).
Target
Function
Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Tissue specificity
Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
Involvement in disease
Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
Sequence similarities
Contains 1 death domain.
Contains 3 TNFR-Cys repeats.
Domain
Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionP25445
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ALPS 1A antibody
- ALPS1A antibody
- APO 1 antibody
see all
Database links
- Entrez Gene: 355 Human
- Entrez Gene: 246097 Rat
- Omim: 134637 Human
- SwissProt: P25445 Human
- SwissProt: Q63199 Rat
- Unigene: 244139 Human
- Unigene: 162521 Rat
Anti-CD95 antibody [IPO-4] images:
Western blot - CD95 antibody [IPO-4] (ab19456)
All lanes : Anti-CD95 antibody [IPO-4] (ab19456) at 5 µg/ml
Lane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/3000 dilution (Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) )
Predicted band size : 45 kDa
Observed band size : 48 kDa (why is the actual band size different from the predicted?)
Additional bands at : 15 kDa,18 kDa. We are unsure as to the identity of these extra bands.
References for Anti-CD95 antibody [IPO-4] (ab19456)
ab19456 has not yet been referenced specifically in any publications.
Publishing research using ab19456? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"