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Western blot - CD95 antibody (ab2437)
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Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - CD95 antibody (ab2437)
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Product Name
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CD95 antibody
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See all CD95 antibodies (37)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to CD95
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Immunogen
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Synthetic peptide mapping near the carboxy terminal domain of human FAS.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Used at a dilution of 1/10 for 30 min on human urothelium and bladder cancer(see Abreview).
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat and A-431 lysate.
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Cellular localization
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Single pass type I membrane protein (isoform 1); Secreted (isoforms 2 to 6).
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Research areas
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Immunology >> Innate Immunity >> Cytokines >> TNF Superfamily
Immunology >> Cell Type Markers >> CD >> Cytokines
Cell Biology >> Apoptosis >> Receptors >> Death Receptors
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Relevance
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CD95, also known as FAS or APO1, is a 36 kDa cell surface type I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS PAGE. CD95 is a member of the TNF receptor family, which includes TNFR1, TNFR2, CD27, CD30 and CD40. Binding of CD95 Ligand to CD95 or crosslinking of CD95 by anti CD95 monoclonal antibodies leads to apoptosis of CD95 expressing cells. CD95 belongs to a subgroup of family members that have a death domain (DD) which contains 70 amino acids near the carboxyl terminal region of the molecule. The binding of adaptor molecules to this DD is responsible for transmitting the death signal for apoptosis. Stimulation of CD95 results in aggregation of its DD, leading to the recruitment of FADD and caspase 8 that together with the receptor form the death inducing signaling complex (DISC). CD95/CD95L is involved in the peripheral deletion of activated mature T cells at the end of the immune response and defects in this pathway predispose to autoimmune disorders. CD95 is also involved in killing of targets such as virus infected cells or cancer cells and killing of inflammatory cells at immune privileged sites.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: 0.2% Gelatin, PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD95 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD95 antibody images, references, products related to ab2437 and other tools.
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CD95 antibody images:
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 Western blot - CD95 antibody (ab2437)
ab2437 at a 1:200 dilution staining Fas in Jurkat whole cell lysate
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Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - CD95 antibody (ab2437)
ab2437 at a 1/10 dilution staining human urothelium (bladder cancer) tissue sections by IHC (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval the tissue was paraformaldehyde fixed and then incubated with the primary antibody for 30 minutes. An HRP conjugated goat antibody was used as the secondary. This image is courtesy of an Abreview submitted by Dr Rudolf Jung
See Abreview
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References for CD95 antibody (ab2437)
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This product has been used in:
Zhang G et al. Vorinostat and sorafenib synergistically kill tumor cells via FLIP suppression and CD95 activation. Clin Cancer Res 14:5385-99 (2008). ICC/IF; Human. PubMed: 18765530
If you publish research using ab2437 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to CD95
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CD95 antibody - more information
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Customer reviews (feedback) regarding CD95 antibody |
Customer FAQs regarding CD95 antibody |
Protocols for CD95 antibody |
Price and availability of products related to CD95 antibody |
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CD95 antibody - related products:
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CD95 antibody - other tools:
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Contact Abcam with a Technical Enquiry about CD95 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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