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All lanes : Anti-CD95 antibody (ab82419) at 1/1000 dilution
Lane 1 : Rat brain tissue extract
Lane 2 : Mouse brain tissue extract
developed using the ECL technique
Predicted band size : 45 kDa
Observed band size : 45 kDa
ab82419 staining CD95 in rat spleen tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in 4% paraformaldehyde, blocked with 10% donkey serum for 20 minutes at 24°C, then incubated with ab82419 at a 1/50 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated donkey anti-rabbit polyclonal, used at a 1/1000 dilution. Counterstained with Hoechst 33258 (blue).
Image courtesy of an anonymous Abreview.
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