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Products:Cardiovascular >> Blood >> Other
Overview
Product name
Anti-CDAN1 antibody
See all CDAN1 products (2) ...
Description
Rabbit polyclonal to CDAN1
Tested applications
ICC/IF, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1150 to the C-terminus of Human Codanin1.
(Peptide available as ab28452.)
Positive control
Hela B Cell Lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Other Antibodies
Cardiovascular >> Blood >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 10 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 130 kDa (predicted molecular weight: 134 kDa).
IHC-P: Use at an assay dependent concentration.
Target
Function
Might be involved in nuclear membrane integrity.
Tissue specificity
Ubiquitously expressed. Isoform 3 is not found in erythroid cells.
Involvement in disease
Defects in CDAN1 are the cause of congenital dyserythropoietic anemia type 1 (CDA1) [MIM:224120]. An autosomal recessive blood disorder characterized by morphological abnormalities of erythroblasts, ineffective erythropoiesis, macrocytic anemia and secondary hemochromatosis. It is occasionally associated with bone abnormalities, especially of the hands and feet (acrodysostosis), nail hypoplasia, and scoliosis. Ultrastructural features include internuclear chromatin bridges connecting some nearly completely separated erythroblasts and an abnormal appearance (spongy or Swiss-cheese appearance) of the heterochromatin in a high proportion of the erythroblasts.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ8IWY9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CDA1 antibody
- CDAI antibody
- CDAN1 antibody
see all
Database links
- Entrez Gene: 146059 Human
- Entrez Gene: 68968 Mouse
- Omim: 607465 Human
- SwissProt: Q8IWY9 Human
- SwissProt: Q3U032 Mouse
- SwissProt: Q8CC12 Mouse
- Unigene: 437819 Human
- Unigene: 599232 Human
see all
Anti-CDAN1 antibody images:
Western blot - Codanin1 antibody (ab28392)
All lanes : Anti-CDAN1 antibody (ab28392) at 1 µg/ml
Lane 1 : Hela B Cell Lysate at 30 µg
Lane 2 : Hela B Cell Lysate at 60 µg
Predicted band size : 134 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Additional bands at : 170 kDa (possible glycosylated form,non-specific binding).
Codanin1 antibody ab28392 detected a band of 130 kDa doublet in Hela B lysate that is likely to correspond to Codanin1. Staining of tubulin in the same lanes is shown as a loading control. The identity of the band at 170 kDa is not known.
The Laboratory of Genevieve Almouzni
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CDAN1 antibody (ab28392)
ab28392 staining CDAN1 in human kidney tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/250 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.
This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
Immunocytochemistry/ Immunofluorescence - Anti-CDAN1 antibody (ab28392)
ICC/IF image of ab28392 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28392, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-CDAN1 antibody (ab28392)
ab28392 has not yet been referenced specifically in any publications.
Publishing research using ab28392? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"