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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab31236? Please let us know so that we can cite the reference in this datasheet
ab31236 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - CDAN1 antibody (ab31236)
All lanes : Anti-CDAN1 antibody (ab31236) at 0.5 µg/ml
Lane 1 : Hela B Cell Lysate at 30 µg
Lane 2 : Hela B Cell Lysate at 60 µg
Predicted band size : 134 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Additional bands at : 170 kDa (possible glycosylated form,non-specific binding).
CDAN1 antibody ab31236 detected a band of 130 kDa doublet in Hela B lysate that is likely to correspond to CDAN1. Staining of tubulin in the same lanes is shown as a loading control. The identity of the band at 170 kDa is not known.
The laboratory of Genevieve Almouzni
Immunocytochemistry/ Immunofluorescence - CDAN1 antibody (ab31236)
ICC/IF image of ab31236 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31236, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Western blot - CDAN1 antibody (ab31236)
Anti-CDAN1 antibody (ab31236) at 1 µg/ml +
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 134 kDa
Observed band size : 134 kDa
Additional bands at : 145 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 minutes
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