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Products:Cardiovascular >> Blood >> Other
Overview
Product name
Anti-CDAN1 antibody
See all CDAN1 products (2) ...
Description
Rabbit polyclonal to CDAN1
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human CDAN1.
(Peptide available as ab28453.)
Positive control
Hela B Cell Lysate, Human Ovary Tissue Lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Other Antibodies
Cardiovascular >> Blood >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 µg/mlDetects a band of approximately 130 kDa (predicted molecular weight: 134 kDa).
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Might be involved in nuclear membrane integrity.
Tissue specificity
Ubiquitously expressed. Isoform 3 is not found in erythroid cells.
Involvement in disease
Defects in CDAN1 are the cause of congenital dyserythropoietic anemia type 1 (CDA1) [MIM:224120]. An autosomal recessive blood disorder characterized by morphological abnormalities of erythroblasts, ineffective erythropoiesis, macrocytic anemia and secondary hemochromatosis. It is occasionally associated with bone abnormalities, especially of the hands and feet (acrodysostosis), nail hypoplasia, and scoliosis. Ultrastructural features include internuclear chromatin bridges connecting some nearly completely separated erythroblasts and an abnormal appearance (spongy or Swiss-cheese appearance) of the heterochromatin in a high proportion of the erythroblasts.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ8IWY9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CDA1 antibody
- CDAI antibody
- CDAN1 antibody
see all
Database links
- Entrez Gene: 146059 Human
- Omim: 607465 Human
- SwissProt: Q8IWY9 Human
- Unigene: 437819 Human
- Unigene: 599232 Human
Anti-CDAN1 antibody images:
Western blot - CDAN1 antibody (ab31236)
All lanes : Anti-CDAN1 antibody (ab31236) at 0.5 µg/ml
Lane 1 : Hela B Cell Lysate at 30 µg
Lane 2 : Hela B Cell Lysate at 60 µg
Predicted band size : 134 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Additional bands at : 170 kDa (possible glycosylated form,non-specific binding).
CDAN1 antibody ab31236 detected a band of 130 kDa doublet in Hela B lysate that is likely to correspond to CDAN1. Staining of tubulin in the same lanes is shown as a loading control. The identity of the band at 170 kDa is not known.
The laboratory of Genevieve Almouzni
Immunocytochemistry/ Immunofluorescence - CDAN1 antibody (ab31236)
ICC/IF image of ab31236 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31236, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Western blot - CDAN1 antibody (ab31236)
Anti-CDAN1 antibody (ab31236) at 1 µg/ml + Ovary (Human) Tissue Lysate - adult normal tissue (ab30222) at 20 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 134 kDa
Observed band size : 134 kDa
Additional bands at : 145 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 minutes
References for Anti-CDAN1 antibody (ab31236)
ab31236 has not yet been referenced specifically in any publications.
Publishing research using ab31236? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"