Overview

  • Product nameAnti-CDC40 antibody
    See all CDC40 primary antibodies
  • Description
    Rabbit polyclonal to CDC40
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized peptide derived from an internal region of human CDC40

  • Positive control
    • Extracts from K562 cells, HUVEC cells or COLO cells

Properties

Applications

Our Abpromise guarantee covers the use of ab74677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 65 kDa.
ELISA 1/40000.

Target

Anti-CDC40 antibody images

  • All lanes : Anti-CDC40 antibody (ab74677) at 1/500 dilution

    Lane 1 : extracts from K562 cells
    Lane 2 : extracts from HUVEC cells
    Lane 3 : extracts from COLO cells
    Lane 4 : extracts from K562 cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 65 kDa
    Observed band size : 65 kDa
    Additional bands at : 22 kDa,34 kDa. We are unsure as to the identity of these extra bands.

References for Anti-CDC40 antibody (ab74677)

ab74677 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Xenopus laevis Cell (egg extract with sperm nuclei)
Specification egg extract with sperm nuclei
Fixative Formaldehyde
Permeabilization Yes - methanol
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 19 2011

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Cell lysate - other (egg extract)
Loading amount 40 µg
Specification egg extract
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 17 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"