Anti-CDC42 binding protein kinase alpha antibody (ab96659)

Overview

  • Product name
    Anti-CDC42 binding protein kinase alpha antibody
    See all CDC42 binding protein kinase alpha primary antibodies
  • Description
    Rabbit polyclonal to CDC42 binding protein kinase alpha
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 373-574 of Human CDC42 binding protein kinase alpha isoform 3 (NP_055641).

  • Positive control
    • H1299, A549 and HeLa whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab96659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 186 kDa.
IHC-P 1/100 - 1/500. Perform heat-mediated antigen retrieval with 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0)

Target

  • Function
    May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.
  • Tissue specificity
    Abundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 CNH domain.
    Contains 1 CRIB domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • Cellular localization
    Cytoplasm. Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent.
  • Information by UniProt
  • Database links
  • Alternative names
    • A930014J19Rik antibody
    • CDC42 binding protein kinase alpha (DMPK like) antibody
    • CDC42 binding protein kinase alpha antibody
    • CDC42 binding protein kinase alpha dmpk like antibody
    • CDC42 binding protein kinase beta antibody
    • CDC42-binding protein kinase alpha antibody
    • CDC42BPA antibody
    • DKFZp686L1738 antibody
    • DKFZp686P1738 antibody
    • DMPK like antibody
    • DMPK-like alpha antibody
    • FLJ23347 antibody
    • KIAA0451 antibody
    • MGC189823 antibody
    • MRCK alpha antibody
    • mrck antibody
    • mrcka antibody
    • MRCKA_HUMAN antibody
    • Myotonic dystrophy kinase related CDC42 binding protein kinase alpha antibody
    • Myotonic dystrophy kinase-related CDC42-binding kinase alpha antibody
    • Myotonic dystrophy protein kinase-like alpha antibody
    • OTTHUMP00000035727 antibody
    • OTTHUMP00000035728 antibody
    • OTTHUMP00000035729 antibody
    • OTTHUMP00000035730 antibody
    • pk428 antibody
    • RP5-1087E8.4 antibody
    • Ser thr protein kinase PK428 antibody
    • Ser thr protein kinase related to the myotonic dystrophy protein kinase antibody
    • Serine/threonine protein kinase MRCK alpha antibody
    • Serine/threonine-protein kinase MRCK alpha antibody
    see all

Images

  • Anti-CDC42 binding protein kinase alpha antibody (ab96659) at 1/1500 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 186 kDa
    7.5% SDS PAGE
  • ab96659 staining CDC42 binding protein kinase alpha in SW480 xenograft at 1/100 dilution by Immunohistochemistry (Formalin/PFA-Fixed Paraffin-embedded sections)

References

This product has been referenced in:
  • Zhu KC  et al. Afzelin exhibits anti-cancer activity against androgen-sensitive LNCaP and androgen-independent PC-3 prostate cancer cells through the inhibition of LIM domain kinase 1. Oncol Lett 10:2359-2365 (2015). WB ; Human . Read more (PubMed: 26622852) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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