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We were looking for an antibody to E-cadherin, whose official gene symbol is CDH1. We found this antibody, and since it uses CDH1 as the main name we thought it was the appropriate antibody. When we got it, the bands were the wrong size for E-cadherin on a Western. Upon further reading the product information sheet, we noticed that the sequence and refseq ID (NP_057347) given are actually for FZR1. While CDH1 has been used as an alias for FZR1, such use is not an approved HUGO gene symbol. Furthermore, nowhere on the product information sheet or the web page (http://www.abcam.com/cdh1-antibody-ab89535.html) does it make reference to these synonyms which would have told us it is the wrong antibody before purchase. Therefore, as we were misled by the product information provided by Abcam, we are requesting a refund of the purchase price or a replacement antibody suitable for staining the real CDH1 (E-cadherin, NCBI GeneID 999) in Western blots. |
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ANSWER: |
The Uniprot link on the datasheet http://www.uniprot.org/uniprot/Q9UM11 is showing FZR1. I understand that this is not enough. I apologize for the misleading information. We are looking into changing the name of the product. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. Your credit note ID is 11938. We have 15 antibodies against E-cadherin which have been tested in WB. I do not know the species of your samples therefore am unable to present you a smaller selection. If you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. I hope this experience will not prevent you from purchasing other products from us in the future. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-FZR1 antibody (ab89535) at 5 µg/ml + immunogen at 0.2 µg
Predicted band size : 55 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Anti-FZR1 antibody (ab89535) at 5 µg/ml + HeLa cell lysate at 50 µg
Predicted band size : 55 kDa
Observed band size : 55 kDa
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