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- CDH1 antibody (ab89535)
- CDH1 antibody (ab89535)
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- CDH1 antibody (ab89535)
- CDH1 antibody (ab89535)
(enlarge)


Product Name 

CDH1 antibody

 

See all CDH1 antibodies (4)...

Product type 

Primary antibodies

Description 

Mouse monoclonal to CDH1

Immunogen 

Recombinant fragment: MDQDYERRLL RQIVIQNENT MPRVTEMRRT LTPASSPVSS PSKHGDRFIP SRAGANWSVN FHRINENEKS PSQNRKAKDA TSDNGKDGLA YSALLKNELL G, corresponding to amino acids 1-102 of human CDH1 (NP_057347), with a 26 kDa proprietary tag.
BLAST 'MDQDYERRLL RQIVIQNENT MPRVTEMRRT LTPASSPVSS PSKHGDRFIP SRAGANWSVN FHRINENEKS PSQNRKAKDA TSDNGKDGLA YSALLKNELL ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 55 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

The immunogen, HeLa cell lysate

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> APC/C

Relevance 

Cdh1 protein is a limiting, substrate-specific activator of anaphase-promoting complex (APC)-dependent proteolysis and it is required for the degradation of the APC substrates, such as Clb2 and Ase1.
Cdh1 plays important role in mitotic regulation.

 

Alternative names CDH1 antibody (ab89535)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

51343    (Human)

NCBI

    

NP_057347    (Human)

Omim

    

603619    (Human)

SwissProt

    

Q9UM11    (Human)

Unigene

    

413133    (Human)

Raised in 

Mouse

Clonality 

Monoclonal

Isotype 

IgG2a

Light chain type 

kappa

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: PBS, pH 7.2

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CDH1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for CDH1 antibody images, references, products related to ab89535 and other tools.

 

CDH1 antibody images:

Product image  - CDH1 antibody (ab89535)

- CDH1 antibody (ab89535)

CDH1 antibody (ab89535) at 5 µg/ml + immunogen at 0.2 µg

Predicted band size : 55 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)

 

Product image  - CDH1 antibody (ab89535)

- CDH1 antibody (ab89535)

CDH1 antibody (ab89535) at 5 µg/ml + HeLa cell lysate at 50 µg

Predicted band size : 55 kDa
Observed band size : 55 kDa

 
 

Search PubMed (MEDLINE) for references to CDH1

 

CDH1 antibody - more information

Customer reviews (feedback) regarding CDH1 antibody

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Protocols for CDH1 antibody

Price and availability of products related to CDH1 antibody

 

CDH1 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for CDH1 antibody

ab14655

  

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

ab29546

  

HeLa (Human epithelial carcinoma cell line) Cytoplasmic Lysate

 

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ab2891

  

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ab6728

  

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Biotin antibody

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Phycoerythrin antibody

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Agarose antibody

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15nm Gold antibody

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10nm Gold antibody

ab27231

  

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ab27233

  

5nm Gold antibody

ab27241

  

10nm Gold antibody

ab27242

  

20nm Gold antibody

ab27243

  

40nm Gold antibody

ab27244

  

5nm Gold antibody

ab60313

  

Chromeo™ 488 antibody

ab60316

  

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Chromeo™ 642 antibody

 

Other CDH1 Antibodies

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CDH1 antibody - other tools:

Contact Abcam with a Technical Enquiry about CDH1 antibody

 

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