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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> APC/C
Overview
Product name
Anti-FZR1 antibody
See all FZR1 products (5) ...
Description
Mouse monoclonal to FZR1
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: MDQDYERRLL RQIVIQNENT MPRVTEMRRT LTPASSPVSS PSKHGDRFIP SRAGANWSVN FHRINENEKS PSQNRKAKDA TSDNGKDGLA YSALLKNELL G, corresponding to amino acids 1-102 of human FZR1 (NP_057347.2), with a 26 kDa proprietary tag.
MDQDYERRLL RQIVIQNENT MPRVTEMRRT LTPASSPVSS PSKHGDRFIP SRAGANWSVN FHRINENEKS PSQNRKAKDA TSDNGKDGLA YSALLKNELL G
Positive control
HeLa cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> APC/C
Applications
Show applications key
Our Abpromise guarantee covers the use of ab89535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 55 kDa.
ELISA: Use at an assay dependent dilution.
Target
Function
Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.
Tissue specificity
Isoform 2 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the WD repeat CDC20/Fizzy family.
Contains 7 WD repeats.
Post-translational
modifications
Phosphorylated during mitosis, probably by maturation promoting factor (MPF), leading to its dissociation of the APC/C. Following DNA damage, it is dephosphorylated by CDC14B in G2 phase, leading to its reassociation with the APC/C, and allowing an efficient G2 DNA damage checkpoint.
Cellular localization
Cytoplasm and Nucleus.
Target information above from: UniProt accessionQ9UM11
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CDC20 like 1b antibody
- CDC20 like protein 1 antibody
- CDC20-like protein 1 antibody
see all
Database links
- Entrez Gene: 51343 Human
- NCBI: 33620742 Human
- Omim: 603619 Human
- SwissProt: Q9UM11 Human
- Unigene: 413133 Human
Anti-FZR1 antibody images:
Western blot - FZR1 antibody (ab89535)
Anti-FZR1 antibody (ab89535) at 5 µg/ml + immunogen at 0.2 µg
Predicted band size : 55 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Western blot - FZR1 antibody (ab89535)
Anti-FZR1 antibody (ab89535) at 5 µg/ml + HeLa cell lysate at 50 µg
Predicted band size : 55 kDa
Observed band size : 55 kDa
References for Anti-FZR1 antibody (ab89535)
ab89535 has not yet been referenced specifically in any publications.
Publishing research using ab89535? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"