Recombinant Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E53] to CDK1 + CDK2 + CDK3 + CDK5
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53]
See all CDK1+CDK2+CDK3+CDK5 primary antibodies -
Description
Rabbit monoclonal [E53] to CDK1 + CDK2 + CDK3 + CDK5 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt or IHC -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Hela cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E53 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa). |
Target
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Cellular localization
CDK1: Nucleus. CDK5: Cytoplasm. Cell projection > lamellipodium. Cell projection > growth cone. In axonal growth cone with extension to the peripheral lamellipodia. -
Database links
- Entrez Gene: 1017 Human
- Entrez Gene: 1018 Human
- Entrez Gene: 1020 Human
- Entrez Gene: 983 Human
- Omim: 116940 Human
- Omim: 116953 Human
- Omim: 123828 Human
- Omim: 123831 Human
see all -
Form
CDK1: CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
Images
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All lanes : Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030) at 1/10000 dilution
Lane 1 : Empty vector transfected 293T (human embryonic kidney) whole cell lysates
Lane 2 : CDK2-GFP transfected 293T (human embryonic kidney) whole cell lysates
Lane 3 : CDK3-GFP transfected 293T (human embryonic kidney) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Diluting buffer 5% NFDM/TBST
The endogenous CDK is detected at higher exposure.
We are not sure the bands around 35kDa. Perhaps they are degradation products.
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All lanes : Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030) at 1/2000 dilution
Lane 1 : Empty vector transfected 293T (human embryonic kidney) whole cell lysates
Lane 2 : CDK1-GFP transfected 293T (human embryonic kidney) whole cell lysates
Lane 3 : CDK5-GFP transfected 293T (human embryonic kidney) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 34 kDa
Exposure time: 15 secondsBlocking/Diluting buffer 5% NFDM/TBST
34kDa band represents endogenous CDK, and 62kDa represents CDK-GFP.
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Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030) at 1/5000 dilution + HeLa cell lysate
Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab32030 has been referenced in 1 publication.
- Ratovitski EA Phospho-?Np63a-dependent microRNAs modulate chemoresistance of squamous cell carcinoma cells to cisplatin: at the crossroads of cell life and death. FEBS Lett 587:2536-41 (2013). PubMed: 23831023