Overview

  • Product nameAnti-CDK1 antibody
    See all CDK1 primary antibodies
  • Description
    Rabbit polyclonal to CDK1
  • Specificityab131450 detects endogenous levels of total CDK1 protein.
  • Tested applicationsWB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, conjugated to KLH, surrounding amino acids 13-17 (G-T-Y-G-V) of Human CDK1 (NP_001163877.1).

  • Positive control
    • Methanol fixed HeLa cells; HeLa and MCF cell extracts; Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab131450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • FunctionPlays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
  • Tissue specificityIsoform 2 is found in breast cancer tissues.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • FormCDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
  • Alternative names
    • Cdc 2 antibody
    • Cdc2 antibody
    • CDC28A antibody
    • CDK 1 antibody
    • CDK1 antibody
    • CDK1_HUMAN antibody
    • CDKN1 antibody
    • CELL CYCLE CONTROLLER CDC2 antibody
    • Cell division control protein 2 antibody
    • Cell division control protein 2 homolog antibody
    • Cell division cycle 2 G1 to S and G2 to M antibody
    • Cell division protein kinase 1 antibody
    • Cell Divsion Cycle 2 Protein antibody
    • Cyclin Dependent Kinase 1 antibody
    • Cyclin-dependent kinase 1 antibody
    • DKFZp686L20222 antibody
    • MGC111195 antibody
    • p34 Cdk1 antibody
    • p34 protein kinase antibody
    • P34CDC2 antibody
    see all

Anti-CDK1 antibody images

  • Immunofluorescent analysis of methanol fixed HeLa cells labelling CDK1 with ab131450 antibody at a dilution of 1/100.
  • All lanes : Anti-CDK1 antibody (ab131450) at 1/500 dilution

    Lane 1 : HeLa cell extracts
    Lane 2 : MCF cell extracts


    Predicted band size : 34 kDa
  • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling CDK1 with ab131450 at 1/50 dilution. The image on the right is treated with the synthesized peptide.

References for Anti-CDK1 antibody (ab131450)

ab131450 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Sample Human Cell lysate - whole cell (293T)
Specification 293T
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Sep 02 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.5% Triton X100
Fixative Paraformaldehyde
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Submitted Sep 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"