Anti-CDK1 antibody (ab131450)

Overview

• Product nameAnti-CDK1 antibodySee all CDK1 primary antibodies ...
• Description
  Rabbit polyclonal to CDK1
• Specificityab131450 detects endogenous levels of total CDK1 protein.
• Tested applicationsWB, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide, conjugated to KLH, surrounding amino acids 13-17 (G-T-Y-G-V) of Human CDK1 (NP_001163877.1).

• Positive controlMethanol fixed HeLa cells; HeLa and MCF cell extracts; Human breast carcinoma tissue.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
  Note: PBS without Mg²⁺ and Ca²⁺
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab131450 was purified by affinity chromatography using epitope specific peptide.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Kinases/Phosphatases
  • Cdks

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Other

  • Neuroscience
  • Neurology process
  • Neural Signal Transduction

  • Cell Biology
  • Cell Cycle
  • Kinases/Phosphatases
  • Cdks

Applications

Target

• FunctionPlays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
• Tissue specificityIsoform 2 is found in breast cancer tissues.
• Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
  Contains 1 protein kinase domain.
• Cellular localizationNucleus.

Target information above from: UniProt accession P06493 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 983 Human
  • Entrez Gene: 983 Human
  • Entrez Gene: 983 Human
  • Entrez Gene: 12534 Mouse
  • Entrez Gene: 54237 Rat
  • Omim: 116940 Human
  • SwissProt: P06493 Human
  • SwissProt: P06493 Human

  • SwissProt: P06493 Human
  • SwissProt: P11440 Mouse
  • SwissProt: P39951 Rat
  • Unigene: 334562 Human
  • Unigene: 281367 Mouse
  • Unigene: 6934 Rat

  see all

• Alternative names
  Cdc 2 antibodyCdc2 antibodyCDC28A antibody

  CDK 1 antibodyCDK1 antibodyCDK1_HUMAN antibodyCDKN1 antibodyCELL CYCLE CONTROLLER CDC2 antibodyCell division control protein 2 antibodyCell division control protein 2 homolog antibodyCell division cycle 2 G1 to S and G2 to M antibodyCell division protein kinase 1 antibodyCell Divsion Cycle 2 Protein antibodyCyclin Dependent Kinase 1 antibodyCyclin-dependent kinase 1 antibodyDKFZp686L20222 antibodyMGC111195 antibodyp34 Cdk1 antibodyp34 protein kinase antibodyP34CDC2 antibody

  see all

Anti-CDK1 antibody images

• 

  Immunocytochemistry/ Immunofluorescence - Anti-CDK1 antibody (ab131450)
  Immunofluorescent analysis of methanol fixed HeLa cells labelling CDK1 with ab131450 antibody at a dilution of 1/100.
• 

  Western blot - Anti-CDK1 antibody (ab131450)
  All lanes : Anti-CDK1 antibody (ab131450) at 1/500 dilution
  
  Lane 1 : HeLa cell extracts
  Lane 2 : MCF cell extracts
  
  
  Predicted band size : 34 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 antibody (ab131450)
  Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling CDK1 with ab131450 at 1/50 dilution. The image on the right is treated with the synthesized peptide.