Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks
Overview
Product name
CDK2 + CCNE1 protein (Tagged)
See all CDK2 + CCNE1 products (2) ...
Protein description
Recombinant full length Human CDK2 (amino acids 1-298) and CCNE1 (amino acids 1-410) with N-terminal proprietary tags co-expressed in a Baculovirus infected Sf9 cell expression system, MWt 58 and 73 kDa respectively (Swiss-Prot P24941 and P24864).
Properties
Purification notes
Purity was determined to be >90% by densitometry.
Form
Liquid
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
Concentration
Concentration information loading...
Research areas
Cancer >> Cell cycle >> Kinases/phosphatases >> Cdks
Cancer >> Cell cycle >> Cyclins >> Cyclin E family
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Epigenetics and Nuclear Signaling >> Cell cycle >> Cyclins >> Cyclin E Family
Cell Biology >> Cell Cycle >> Cyclins >> Cyclin E Family
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks
-
SDS-PAGE - CDK2 + CCNE1 protein (Tagged) (ab85836)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab85836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
Protein info
Relevance
CDK2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. CCNE1 (Cyclin E) is a regulatory subunit of CDK2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
Cellular localization
CCNE1: Nuclear.
CDK2 + CCNE1 protein (Tagged) images:
SDS-PAGE - CDK2 + CCNE1 protein (Tagged) (ab85836)
SDS-PAGE showing ab85836 at approximately 58 kDa (CDK2) and 73 kDa (CCNE1).
References for CDK2 + CCNE1 protein (Tagged) (ab85836)
ab85836 has not yet been referenced specifically in any publications.
Publishing research using ab85836? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"