Anti-CDK5RAP1 antibody (ab154537)
Key features and details
- Rabbit polyclonal to CDK5RAP1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CDK5RAP1 antibody -
Description
Rabbit polyclonal to CDK5RAP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
A recombinant fragment corresponding to a region within amino acids 305- 590 of Human CDK5RAP1 (Uniprot ID Q96SZ6).
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Positive control
- HeLa whole cell lysate. Human breast carcinoma tissue. HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 68 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 68 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Relevance
CDK5RAP1 specifically inhibits CDK5 activation by CDK5R1. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 51654 Human
- Entrez Gene: 66971 Mouse
- Entrez Gene: 252827 Rat
- Omim: 608200 Human
- SwissProt: Q96SZ6 Human
- SwissProt: Q8BTW8 Mouse
- SwissProt: Q9JLH6 Rat
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Alternative names
- 2310066P17Rik antibody
- C20orf34 antibody
- C42 antibody
see all
Images
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Anti-CDK5RAP1 antibody (ab154537) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 68 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling CDK5RAP1 using ab154537 at 1/500 dilution. Right panel costained with Hoechst 33342.
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Immunohistochemical analysis of paraffin-embedded Human breast carcinoma, labeling CDK5RAP1 using ab154537 at 1/250 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154537 has not yet been referenced specifically in any publications.