CDKN2A/p16INK4a antibody [2D9A12] (ab54210)

Product Name 

CDKN2A/p16INK4a antibody [2D9A12]

Product type 

Primary antibodies

Description 

Mouse monoclonal [2D9A12] to CDKN2A/p16INK4a

Immunogen 

Ni-NTA purified recombinant P16 expressed in E. Coli strain BL21 (DE3).

Reacts with 
(species key)

Hu, Rat

Tested applications 
(see key)

ELISA, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: 1/10000.
IHC-P: 1/200 - 1/1000. Use at an assay dependent dilution. Perform heat mediated antigen retrieval in citrate buffer pH 6.0 for 2 min only via the pressure cooker method before commencing with IHC staining protocol. A signal enhancing step could be necessary.
WB: 1/500 - 1/2000 - tested using a recombinant protein expressed in E. coli. We have no data on the detection of the endogenous protein and would welcome feedback from anyone that has performed a WB with ab54210 using cell lysates.



Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Rat liver tissue; Human brain tissue; Human brain tumor tissue.

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> INK4a/Arf
Cancer >> Cell cycle >> Cell cycle inhibitors >> Ink4
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Ink4
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Ink4

Relevance 

The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

2D9A12

Isotype 

IgG2b

Purity 

Ascites

Storage buffer 

Preservative: 0.03% Sodium Azide
Constituents: Ascites

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

See below for CDKN2A/p16INK4a antibody [2D9A12] images, references, products related to ab54210 and other tools.

CDKN2A/p16INK4a antibody [2D9A12] images:

References for CDKN2A/p16INK4a antibody [2D9A12] (ab54210)

  This product has been used in: (two most recent references)

Almeida MQ et al. Mouse Prkar1a haploinsufficiency leads to an increase in tumors in the Trp53+/- or Rb1+/- backgrounds and chemically induced skin papillomas by dysregulation of the cell cycle and Wnt signaling. Hum Mol Genet 19:1387-98 (2010). IHC-P; Mouse. PubMed: 20080939

Wang GS et al. Enhanced islet expansion by beta-cell proliferation in young diabetes-prone rats fed a protective diet. J Cell Physiol 224:501-8 (2010). IHC-P; Rat. PubMed: 20432450
If you publish research using ab54210 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding CDKN2A/p16INK4a antibody [2D9A12]

Customer FAQs regarding CDKN2A/p16INK4a antibody [2D9A12]

Protocols for CDKN2A/p16INK4a antibody [2D9A12]

Price and availability of products related to CDKN2A/p16INK4a antibody [2D9A12]

CDKN2A/p16INK4a antibody [2D9A12] - related products: