Anti-CDKN2A/p16INK4a antibody [2D9A12] (ab54210)

Overview

• Product nameAnti-CDKN2A/p16INK4a antibody [2D9A12]See all CDKN2A/p16INK4a primary antibodies ...
• Description
  Mouse monoclonal [2D9A12] to CDKN2A/p16INK4a
• Tested applicationsFlow Cyt, WB, ELISA, IHC-P more details
• Species reactivity
  Reacts with: Rat, Human
  
• Immunogen

  Ni-NTA purified recombinant P16 expressed in E. Coli strain BL21 (DE3).

• Positive controlRat liver tissue; Human brain tissue; Human brain tumor tissue.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.03% Sodium Azide
  Constituents: Ascites
• Concentration information loading...
• PurityAscites
• Clonality Monoclonal
• Clone number2D9A12
• IsotypeIgG2b
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • INK4a/Arf

  • Cancer
  • Cell cycle
  • Cell cycle inhibitors
  • Ink4

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cell Cycle Inhibitors
  • Ink4

  • Cell Biology
  • Cell Cycle
  • Cell Cycle Inhibitors
  • Ink4

Applications

Target

• RelevanceThe CDKN2A locus gives rise to 2 distinct transcripts from different promoters. The transcripts have been designated p16(INK4A) and p14(ARF). This chromosomal region undergoes a number of inversions, translocations, heterozygous deletions, and homozygous deletions in a variety of malignant cell lines including those from glioma, non-small cell lung cancer, leukemia, and melanoma. Deletion of the region containing CDKN2A is found in more than half of all melanoma cell lines. In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
• Cellular localizationCytoplasmic and Nuclear
• Database links
  • Entrez Gene: 1029 Human
  • Entrez Gene: 25163 Rat
  • Omim: 600160 Human
  • SwissProt: P42771 Human
  • SwissProt: Q9R0Z3 Rat
  • Unigene: 512599 Human
  • Unigene: 48717 Rat

• Alternative names
  ARF antibodyCCM2 antibodyCDK4 Inhibitor antibody

  CDK4 inhibitor p16 INK4 antibodyCDK4I antibodyCDKN2 antibodyCDKN2A antibodyCell cycle negative regulator beta antibodyCMM2 antibodyCyclin dependent kinase 4 inhibitor A antibodyCyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibodyCyclin Dependent Kinase Inhibitor 2A antibodyCyclin dependent kinase inhibitor 2A isoform 4 antibodyCyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibodyCyclin dependent kinase inhibitor p16 antibodyINK4 antibodyINK4 antibodyINK4A antibodyMLM antibodyMTS1 antibodyMultiple tumor suppressor 1 antibodyMultiple Tumor Supressor 1 antibodyp14 antibodyp14(ARF) antibodyp14ARF antibodyp16 antibodyp16 INK4 antibodyp16INK4 antibodyP16INK4 antibodyp16INK4a antibodyp19 antibodyp19(ARF) antibodyp19Arf antibodyTP16 antibody

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Anti-CDKN2A/p16INK4a antibody [2D9A12] images

• 

  Western blot - p16 INK4a antibody [2D9A12] (ab54210)
  All lanes : Anti-CDKN2A/p16INK4a antibody [2D9A12] (ab54210) at 1/500 dilution
  
  Lane 1 : Molecular weight marker
  Lane 2 : Truncated p16 recombinant protein
  
  
  Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - CDKN2A/p16INK4a antibody [2D9A12] (ab54210)
  Ab54210 (1:500) staining p16 INK4a in rat liver tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (by DAB staining) is shown.
• 

  Immunohistochemistry (Paraffin-embedded sections) - CDKN2A/p16INK4a antibody [2D9A12] (ab54210)
  Ab54210 (1:500) staining p16 INK4a in human brain tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (by DAB staining) is shown.
• 

  Immunohistochemistry (Paraffin-embedded sections) - CDKN2A/p16INK4a antibody [2D9A12] (ab54210)
  Ab54210 (1:500) staining p16 INK4a in human brain tumor tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (by DAB staining) is shown.
• 

  Flow Cytometry - Anti-CDKN2A/p16INK4a antibody [2D9A12] (ab54210)
  Overlay histogram showing HeLa cells stained with ab54210 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54210, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.