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Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer pH: 7.40 Preservative: 0.02% Sodium azide Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 17 kDa.
1/50 - 1/100.
1/100 - 1/500.
Relevance The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
Cellular localization Cytoplasmic and Nuclear
CDK4 inhibitor p16 INK4 antibody
Anti-CDKN2A/p14ARF antibody images
Western blot - Anti-CDKN2A/p14ARF antibody (ab129755)
All lanes : Anti-CDKN2A/p14ARF antibody (ab129755) at 1/500 dilution Lane 1 : HeLa cell extract Lane 2 : HeLa cell extract with immunizing peptide Predicted band size : 17 kDa
Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p14ARF antibody (ab129755)
ab129755, at 1/100 dilution, staining CDKN2A/p14ARF in HeLa cells by Immunofluorescence, in the presence (right panel) or absence (left panel) of immunizing peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p14ARF antibody (ab129755)
ab129755, at 1/50 dilution, staining CDKN2A/p14ARF in paraffin-embedded Human placenta tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunizing peptide.
References for Anti-CDKN2A/p14ARF antibody (ab129755)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"