Anti-CDKN2A/p14ARF antibody (ab129755)

Overview

• Product nameAnti-CDKN2A/p14ARF antibodySee all CDKN2A/p14ARF primary antibodies ...
• Description
  Rabbit polyclonal to CDKN2A/p14ARF
• Tested applicationsWB, ELISA, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide derived from Human CDKN2A/p14ARF (UniProt Q8N726).

• Positive controlHuman placenta tissue; HeLa cells; HeLa cell extract.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • p53 pathway

  • Cancer
  • Cell cycle
  • Cell cycle inhibitors
  • Ink4

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Cancer susceptibility
  • Tumor Suppressors

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cell Cycle Inhibitors
  • Ink4

  • Cell Biology
  • Cell Cycle
  • Cell Cycle Inhibitors
  • Ink4

Applications

Target

• RelevanceThe gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
• Cellular localizationCytoplasmic and Nuclear
• Database links
  • Entrez Gene: 1029 Human
  • Omim: 600160 Human
  • SwissProt: P42771 Human
  • SwissProt: Q8N726 Human
  • Unigene: 512599 Human

• Alternative names
  ARF antibodyCDK4 inhibitor p16 INK4 antibodyCDK4I antibody

  CDKN2 antibodyCDKN2A antibodyCell cycle negative regulator beta antibodyCMM2 antibodyCyclin dependent kinase 4 inhibitor A antibodyCyclin dependent kinase inhibitor 2A antibodyINK4 antibodyINK4a antibodyMelanoma p16 inhibits CDK4 antibodyMLM antibodyMTS 1 antibodyMTS1 antibodyMultiple tumor suppressor 1 antibodyp14 antibodyp16 antibodyp16 INK4a antibodyp16INK4a antibodyp19 antibodyP19ARF antibodyTP16 antibody

  see all

Anti-CDKN2A/p14ARF antibody images

• 

  Western blot - Anti-CDKN2A/p14ARF antibody (ab129755)
  All lanes : Anti-CDKN2A/p14ARF antibody (ab129755) at 1/500 dilution
  
  Lane 1 : HeLa cell extract
  Lane 2 : HeLa cell extract with immunizing peptide
  
  
  Predicted band size : 17 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p14ARF antibody (ab129755)
  ab129755, at 1/100 dilution, staining CDKN2A/p14ARF in HeLa cells by Immunofluorescence, in the presence (right panel) or absence (left panel) of immunizing peptide.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p14ARF antibody (ab129755)
  ab129755, at 1/50 dilution, staining CDKN2A/p14ARF in paraffin-embedded Human placenta tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunizing peptide.