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Overview

  • Product nameAnti-CDKN2A/p16INK4a antibodySee all CDKN2A/p16INK4a primary antibodies ...
  • Description
    Rabbit polyclonal to CDKN2A/p16INK4a
  • SpecificityReacts with p16
  • Tested applicationsICC/IF, IHC-P, IP, WB, Sandwich ELISA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) (C terminal).

  • Positive controlHeLa

Properties

Applications

Our Abpromise guarantee covers the use of ab7962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: Use a concentration of 1 µg/ml.
IHC-P IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
IP IP: Use at an assay dependent dilution.
WB WB: 1/200. Predicted molecular weight: 16 kDa.Can be blocked with p16 (C-term) peptide (human) (ab8017).
Sandwich ELISA sELISA: Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [DCS50.1] to CDKN2A/p16INK4a (ab16123). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [DCS50.1] to CDKN2A/p16INK4a (ab16123) as Capture.

Target

  • RelevanceThe CDKN2A locus gives rise to 2 distinct transcripts from different promoters. The transcripts have been designated p16(INK4A) and p14(ARF). This chromosomal region undergoes a number of inversions, translocations, heterozygous deletions, and homozygous deletions in a variety of malignant cell lines including those from glioma, non-small cell lung cancer, leukemia, and melanoma. Deletion of the region containing CDKN2A is found in more than half of all melanoma cell lines. In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • FormThere are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF.
    • Alternative names
        ARF antibodyCCM2 antibodyCDK4 Inhibitor antibody
        CDK4 inhibitor p16 INK4 antibodyCDK4I antibodyCDKN2 antibodyCDKN2A antibodyCell cycle negative regulator beta antibodyCMM2 antibodyCyclin dependent kinase 4 inhibitor A antibodyCyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibodyCyclin Dependent Kinase Inhibitor 2A antibodyCyclin dependent kinase inhibitor 2A isoform 4 antibodyCyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibodyCyclin dependent kinase inhibitor p16 antibodyINK4 antibodyINK4 antibodyINK4A antibodyMLM antibodyMTS1 antibodyMultiple tumor suppressor 1 antibodyMultiple Tumor Supressor 1 antibodyp14 antibodyp14(ARF) antibodyp14ARF antibodyp16 antibodyp16 INK4 antibodyp16INK4 antibodyP16INK4 antibodyp16INK4a antibodyp19 antibodyp19(ARF) antibodyp19Arf antibodyTP16 antibody
      see all

    Anti-CDKN2A/p16INK4a antibody images

    • Sandwich ELISA - CDKN2A/p16INK4a antibody (ab7962)
      Sandwich ELISA - CDKN2A/p16INK4a antibody (ab7962)
      Standard Curve for CDKN2A/p16INK4a (Analyte: CDKN2A/p16INK4a protein (Human) (ab84075)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [DCS50.1] to CDKN2A/p16INK4a (ab16123) at 1µg/ml and Detector Antibody Rabbit polyclonal to CDKN2A/p16INK4a (ab7962) at 0.5µg/ml.
    • Immunocytochemistry/ Immunofluorescence-CDKN2A/p16INK4a antibody(ab7962)
      Immunocytochemistry/ Immunofluorescence-CDKN2A/p16INK4a antibody(ab7962)
      ICC/IF image of ab7962 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7962, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    References for Anti-CDKN2A/p16INK4a antibody (ab7962)

    This product has been referenced in:
    • Tsoumpou I  et al. p16(INK4a) immunostaining in cytological and histological specimens from the uterine cervix: a systematic review and meta-analysis. Cancer Treat Rev 35:210-20 (2009). ICC, IHC ; Human . Read more (PubMed: 19261387) »

    See 1 Publication for this product

    Publishing research using ab7962 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab7962 Submit a Question for ab7962

    Displaying 1 - 4 of 4 results for Abreviews and Q&A

    10
    Votes: Highest First

    Dear xxx
    thanks for reply and information.. we are planing to perform immunohistochemistryfor paraffin section (IHC-P).

    Best regards

    Sorry for the delay in getting back to you. I wanted to make sure I had all the information I required in order to make the most suitable suggestions before getting back to you.


    From my search these are the antibodies I can suggest to you:...

    Read More

    The customer tested same human sample with ab7962 and ab54210 but two results showed different pattern.
    ab7962 showed 16, 40 kDa bands but 16 kDa is more strong and
    ab54210 also showed two bands but 40 kDa is more storng.
    If 16kDa is from i...

    Read More

    I apologise for keeping you waiting.

    I heard back from my lab colleagues, and confirmed the results you had obtained are consistent with the specificity of both antibodies.

    Ab54210 recognises isoform 4 of the protein, at ˜40KDa, wherea...

    Read More

    Phone call: Customer kindly requested IHC image of ab7962 with DAB staining.

    Thank you for contacting us. Please find attached the IHC-P protocol and image you requested. DAB was used in IHC-P. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

    I ordered the p16 - ab7962 antibody last week on 9 July (reference number 8961), and have found puzzling results after using it in a Western blot. Though the size of the protein detected should be 16 kD, I have found something more around the size of 35 ...

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    As promised, here is the response from the originator of this antibody: "I have looked at our WB for ab7962and while I do see a protein around 35 kD (more like 32kD), I still am getting a band at 16 kD in the HeLa lysates. I would say that they are of ...

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