Overview

  • Product nameAnti-CDKN2A/p16INK4a antibodySee all CDKN2A/p16INK4a primary antibodies ...
  • Description
    Rabbit polyclonal to CDKN2A/p16INK4a
  • SpecificityReacts with p16
  • Tested applicationsICC/IF, IHC-P, IP, WB, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) (C terminal).

  • Positive control
    • HeLa

Properties

Applications

Our Abpromise guarantee covers the use of ab7962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
IP Use at an assay dependent dilution.
WB 1/200. Predicted molecular weight: 16 kDa.Can be blocked with CDKN2A/p16INK4a peptide (ab8017).
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [DCS50.1] to CDKN2A/p16INK4a (ab16123). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [DCS50.1] to CDKN2A/p16INK4a (ab16123) as Capture.

Target

  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • FormThere are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF.
  • Alternative names
    • ARF antibody
    • CCM2 antibody
    • CDK4 Inhibitor antibody
    • CDK4 inhibitor p16 INK4 antibody
    • CDK4I antibody
    • CDKN2 antibody
    • CDKN2A antibody
    • Cell cycle negative regulator beta antibody
    • CMM2 antibody
    • Cyclin dependent kinase 4 inhibitor A antibody
    • Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody
    • Cyclin Dependent Kinase Inhibitor 2A antibody
    • Cyclin dependent kinase inhibitor 2A isoform 4 antibody
    • Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody
    • Cyclin dependent kinase inhibitor p16 antibody
    • INK4 antibody
    • INK4 antibody
    • INK4A antibody
    • MLM antibody
    • MTS1 antibody
    • Multiple tumor suppressor 1 antibody
    • Multiple Tumor Supressor 1 antibody
    • p14 antibody
    • p14(ARF) antibody
    • p14ARF antibody
    • p16 antibody
    • p16 INK4 antibody
    • p16INK4 antibody
    • P16INK4 antibody
    • p16INK4a antibody
    • p19 antibody
    • p19(ARF) antibody
    • p19Arf antibody
    • TP16 antibody
    see all

Anti-CDKN2A/p16INK4a antibody images

  • Standard Curve for CDKN2A/p16INK4a (Analyte: CDKN2A/p16INK4a protein (Human) (ab84075)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [DCS50.1] to CDKN2A/p16INK4a (ab16123) at 1µg/ml and Detector Antibody Rabbit polyclonal to CDKN2A/p16INK4a (ab7962) at 0.5µg/ml.
  • ICC/IF image of ab7962 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7962, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Protocols

References for Anti-CDKN2A/p16INK4a antibody (ab7962)

This product has been referenced in:
  • Balsat C  et al. Improved computer-assisted analysis of the global lymphatic network in human cervical tissues. Mod Pathol N/A:N/A (2013). Human . Read more (PubMed: 24309324) »
  • Tsoumpou I  et al. p16(INK4a) immunostaining in cytological and histological specimens from the uterine cervix: a systematic review and meta-analysis. Cancer Treat Rev 35:210-20 (2009). ICC, IHC ; Human . Read more (PubMed: 19261387) »

See all 2 Publications for this product

Product Wall

Sorry for the delay in getting back to you. I wanted to make sure I had all the information I required in order to make the most suitable suggestions before getting back to you.


From my search these are the antibodies I can suggest to y...

Read More

I apologise for keeping you waiting.

I heard back from my lab colleagues, and confirmed the results you had obtained are consistent with the specificity of both antibodies.

Ab54210 recognises isoform 4 of the protein, at ˜40KDa, whe...

Read More

Thank you for contacting us. Please find attached the IHC-P protocol and image you requested. DAB was used in IHC-P. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

As promised, here is the response from the originator of this antibody: "I have looked at our WB for ab7962and while I do see a protein around 35 kD (more like 32kD), I still am getting a band at 16 kD in the HeLa lysates. I would say that they are ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"