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Anti-CDKN3 antibody [2H10] (ab119242)

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Overview

  • Product nameAnti-CDKN3 antibody [2H10]See all CDKN3 primary antibodies ...
  • Description
    Mouse monoclonal [2H10] to CDKN3
  • Tested applicationsWB, Flow Cyt more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length recombinant Human CDKN3 (NP_005183) produced in HEK293T cells.

  • Positive controlHEK293T cells transfected with pCMV6-ENTRY CDKN3.

Properties

Applications

Our Abpromise guarantee covers the use of ab119242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/2000. Predicted molecular weight: 24 kDa.
Flow Cyt Flow Cyt: 1/100.

Target

  • FunctionMay play a role in cell cycle regulation. Dual specificity phosphatase active toward substrates containing either phosphotyrosine or phosphoserine residues. Dephosphorylates CDK2 at 'Thr-160' in a cyclin-dependent manner.
  • Involvement in diseaseDefects in CDKN3 are found in patients with hepatocellular carcinoma (HCC) [MIM:114550].
  • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family.
  • Cellular localizationCytoplasm > perinuclear region.

    • Target information above from: UniProt accession Q16667 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        CDI1 antibodyCdk associated protein phosphatase antibodyCDK2 associated dual specificity phosphatase antibody
        CDK2-associated dual-specificity phosphatase antibodyCDKN3 antibodyCDKN3_HUMAN antibodyCIP2 antibodyCyclin dependent kinase inhibitor 3 antibodyCyclin dependent kinase interacting protein 2 antibodyCyclin dependent kinase interactor 1 antibodyCyclin-dependent kinase inhibitor 3 antibodyCyclin-dependent kinase interactor 1 antibodyCyclin-dependent kinase-interacting protein 2 antibodyFLJ25787 antibodyKAP antibodyKAP1 antibodyKinase associated phosphatase antibodyKinase-associated phosphatase antibodyMGC70625 antibodyOTTHUMP00000178991 antibody
      see all

    Anti-CDKN3 antibody [2H10] images

    • Western blot - Anti-CDKN3 antibody [2H10] (ab119242)
      Western blot - Anti-CDKN3 antibody [2H10] (ab119242)
      All lanes : Anti-CDKN3 antibody [2H10] (ab119242) at 1/2000 dilution

      Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control
      Lane 2 : HEK293T cells transfected with pCMV6-ENTRY CDKN3

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 24 kDa
    • Flow Cytometry - Anti-CDKN3 antibody [2H10] (ab119242)
      Flow Cytometry - Anti-CDKN3 antibody [2H10] (ab119242)
      Flow cytometric analysis of HEK293T cells transfected with either CDKN3 overexpress plasmid (Red) or empty vector control plasmid (Blue) labelled with ab119242 at 1/100 dilution.

    References for Anti-CDKN3 antibody [2H10] (ab119242)

    ab119242 has not yet been referenced specifically in any publications.

    Publishing research using ab119242 ? Please let us know so that we can cite the reference in this datasheet

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    Hello, We have tried to test two of your new CDKN3 antibodies (catalog numbers above) and unfortunately we have not been successful. We had been using another CDKN3 antibody from Abcam (ab64974) for years and it has consistently worked well, but now it...

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    Thank you for contacting us. I am sorry that the other CDNK3 products have not been acceptable in your assays and that you have been experiencing issues when using ab119242. This product would be able to detect endogenous CDKN3, the cell line chosen for...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"