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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
This fast track antibody is not yet fully characterised. It is subject to these terms and conditions
Overview
Product name
Anti-CDON / Cdo antibody
Description
Rabbit polyclonal to CDON / Cdo
This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
Cross reactivity
Predicted to work with
Mouse, Human
Immunogen
Synthetic peptide derived from within residues 950 - 1050 of Human CDON / Cdo.
(Peptide available as ab34188.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Hedgehog >> Surface Molecules
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
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Immunocytochemistry/ Immunofluorescence - CDON / Cdo antibody (ab34189)
(enlarge)
Applications
Application notes
This antibody gave a positive result in ELISA against the immunizing peptide (ab34188).
Not yet tested in other applications.
Target
Relevance
CDON (Cdo), which is implicated in myogenesis also has a role in human forebrain development. Holoprosencephaly (HPE), a common defect of human forebrain development, is associated with haploinsufficiency for genes encoding Sonic Hedgehog (SHH) pathway components. Cdo positiviely regulates SHH in multiple ways, including at signal reception and via a parallel mechanism required at the level of Gli transcription factors. Specific Cdo domains required for its promyogenic effect are dispensable for its Shh signaling role, suggesting that Cdo has multiple, independent functions.
Cellular localization
Cell Membrane
Alternative names
- CDO antibody
- CDON antibody
- Cell adhesion molecule-related/down-regulated by oncogenes antibody
Database links
- Entrez Gene: 50937 Human
- Entrez Gene: 57810 Mouse
- Omim: 608707 Human
- SwissProt: Q4KMG0 Human
- SwissProt: Q32MD9 Mouse
- Unigene: 38034 Human
- Unigene: 596333 Human
- Unigene: 80509 Mouse
Anti-CDON / Cdo antibody images:
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Immunocytochemistry/ Immunofluorescence - CDON / Cdo antibody (ab34189)
ICC/IF image of ab34189 stained human MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab34189, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and HepG2 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
References for Anti-CDON / Cdo antibody (ab34189)
ab34189 has not yet been referenced specifically in any publications.
Publishing research using ab34189? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"