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Product Name
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CDON / Cdo antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to CDON / Cdo
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Note on antibody grade |
Fast-Track antibodies
- Immunogen affinity purified
- high ELISA titre values
- released early (to accelerate your research)
- 950 Abpoints to the 1st researcher
who submits conclusive data (positive or negative):
Read the Fast-Track antibody Terms of Use
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Immunogen
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Synthetic peptide derived from within residues 950 - 1050 of Human CDON / Cdo. (Peptide available as ab34188.)
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Predicted to react
(species key)
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Hu
(Due to sequence homology)
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Abreviews
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Application notes
(see key)
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Recommended dilutions
This antibody gave a positive result in ELISA against the immunizing peptide (ab34188).
Not yet tested in other applications.
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Cellular localization
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Cell Membrane
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Research areas
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Stem Cells >> Signaling Pathways >> Hedgehog >> Surface Molecules
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
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Relevance
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CDON (Cdo), which is implicated in myogenesis also has a role in human forebrain development. Holoprosencephaly (HPE), a common defect of human forebrain development, is associated with haploinsufficiency for genes encoding Sonic Hedgehog (SHH) pathway components. Cdo positiviely regulates SHH in multiple ways, including at signal reception and via a parallel mechanism required at the level of Gli transcription factors.
Specific Cdo domains required for its promyogenic effect are dispensable for its Shh signaling role, suggesting that Cdo has multiple, independent functions.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.300 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CDON / Cdo antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CDON / Cdo antibody images, references, products related to ab34189 and other tools.
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CDON / Cdo antibody images:
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This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Please read the definition of 'Fast-Track antibody'
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 Immunocytochemistry/ Immunofluorescence - CDON / Cdo antibody (ab34189)
ICC/IF image of ab34189 stained human MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab34189, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and HepG2 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
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Search PubMed (MEDLINE) for references to CDON / Cdo
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CDON / Cdo antibody - more information
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Customer reviews (feedback) regarding CDON / Cdo antibody |
Customer FAQs regarding CDON / Cdo antibody |
Protocols for CDON / Cdo antibody |
Price and availability of products related to CDON / Cdo antibody |
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CDON / Cdo antibody - related products:
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CDON / Cdo antibody - other tools:
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Contact Abcam with a Technical Enquiry about CDON / Cdo antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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