Anti-CDT1 antibody (ab77091)

Overview

• Product nameAnti-CDT1 antibodySee all CDT1 primary antibodies ...
• Description
  Goat polyclonal to CDT1
• Tested applicationsWB, ELISA more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide: ARLAHQTRAEEGL by a Cysteine residue linker, corresponding to C terminal amino acids 534-546 of Human CDT1

  Run BLAST with Run BLAST with
• Positive controlHuman brain lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Synthesis
  • Other

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Cancer susceptibility
  • Proto-oncogenes

Applications

Target

• FunctionCooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene.
• Sequence similaritiesBelongs to the Cdt1 family.
• Developmental stagePresent during G1 and early S phase of the cell cycle. Degraded during the late S, G2, and M phases.
• DomainThe PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
• Post-translational
  modificationsUbiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, in response to DNA damage, leading to its degradation. Ubiquitination by the DCX(DTL) complex is necessary to ensure proper cell cycle regulation and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. The interaction with GMNN protects it against ubiquitination.
  Phosphorylated by cyclin A-dependent kinases which results in the binding of CDT1 to the F-box protein SKP2 and subsequent degradation. Binding to GMNN is not affected by phosphorylation.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q9H211 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 81620 Human
  • Omim: 605525 Human
  • SwissProt: Q9H211 Human
  • Unigene: 122908 Human

• Alternative names
  CDT 1 antibodycdt1 antibodyCDT1_HUMAN antibody

  Chromatin licensing and DNA replication factor 1 antibodyDNA replication factor antibodyDNA replication factor Cdt1 antibodyDouble parked antibodyDouble parked Drosophila homolog of antibodyDouble parked homolog antibodyDUP antibodyRetroviral integration site 1 antibodyRetroviral integration site 2 antibodyRetroviral integration site1 antibodyRetroviral integration site2 antibodyRIS 2 antibodyRIS2 antibody

  see all

Anti-CDT1 antibody images

• 

  Western blot - CDT1 antibody (ab77091)
  Anti-CDT1 antibody (ab77091) at 1 µg/ml + Human brain lysate at 35 µg
  developed using the ECL technique
  
  Predicted band size : 63 kDa
  Observed band size : 63 kDa