CEACAM 5 + 6 antibody [MUS] - Azide free (ab4539)

Product Name 

CEACAM 5 + 6 antibody [MUS] - Azide free

Product type 

Primary antibodies

Description 

Mouse monoclonal [MUS] to CEACAM 5 + 6 - Azide free

Immunogen 

Full length native Carcino Embryonic Antigen(partially purified) (Human) from a perchloric acid extract from liver metastases of colonic tumors (Schozel S et al.).

Reacts with 
(species key)

Hu

Specificity 

MUS reacts specifically with CEACAM 5 (CEA/CD66e) and CEACAM 6 (NCA/CD66c) transiently expressed on the cell surface of transfected BOSC23 cells as demonstrated by flow cytometry.

Tested applications 
(see key)

ELISA, Flow Cyt, IHC-Fr, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependant dilution.
Flow Cyt: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution. Predicted molecular weight Carcino Embryonic Antigen: 84 kDa. Predicted molecular weight CEACAM 6: 41 kDa

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Plasma membrane

Research areas 

Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

Relevance 

CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glycoprotein (PSG1-PSG11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. CEACAM 6 expression is strongly up-regulated already during early stages of adenocarcinoma formation e.g. in colon. The function of CEA family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

MUS

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: None
Constituents: PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C.

Notes 

Antibodies produced from cDNA:

Conventional technologies usually either generate antibodies against purified proteins, or against synthetic peptides based on amino acid sequences derived from DNA sequence data. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein.

Although the synthetic peptide approach is comparable in speed, the quality of antibodies generated by genetic immunization is far superior. This is because the protein is made by the immunized animal, utilzing complex cellular mechanisms that allow it to gain a native conformation. Antibodies are then generated against a native protein, such as is found in the blood or tissues of its host species. Membrane-bound or secreted proteins often create problems for conventional antibody technology because in their native form, they are often modified by glycosylation, or in some cases exist as multiple membrane-spanning proteins that are not soluble following isolation or synthesis in recombinant systems. All of these problems are avoided if the immunized animal makes the protein itself.

Antibodies generated by genetic immunization have been shown to have binding affinities to the protein in the sub-nanomolar range, which are approximately 100x higher than conventionally developed antibodies and much higher than single chain antibodies. Results confirm published data for much higher avidity of sera generated by genetic immunization as compared with that gained by immunization with a corresponding recombinant protein.

References for CEACAM 5 + 6 antibody [MUS] - Azide free (ab4539)

  This product has been used in:

Tsutsumida H et al. RNA interference suppression of MUC1 reduces the growth rate and metastatic phenotype of human pancreatic cancer cells. Clin Cancer Res 12:2976-87 (2006). PubMed: 16707592

If you publish research using ab4539 please let us know so that we can cite the reference on this datasheet.

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