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AbID: 56234, Anti-CEACAM6 antibody Rating: Poor Image: Anti-CEACAM6 antibody for Immunohistochemistry (Frozen sections) (Human) Sample: Species: Human Type: Tissue sections Specification: Human primary breast cancer Application: Application: Fixative: Methanol Blocking step: Serum as blocking agent for 10 mins at 25°C Other detail: Dilution: 1/100 Incubation time: 1 hour at 25°C Diluent: PBS + 10% FCS Secondary Antibody: Name: Non-Abcam Antibody was used: UltraVision ONE HRP system Conjugation: HRP Additional Data: Additional Notes: No specific reaction whatsoever. I tested this antibody along with three other CEACAM6 antibodies (including 9A6 from abcam) on the same tissue sections, and only this one did not test positive. |
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ANSWER: |
Thank you for submitting your review. I hope you have received notification that it has been "published" to the datasheet for ab56234 on our website. I am sorry to hear the antibody did not perform as expected. If you have received this vial of ab56234 within 6 months or so of submitting your review I would be happy to offer a replacement, credit or refund. In your reply, please include the purchase order number or Abcam order reference number associated with this purchase so I can process your request. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-CEACAM6 antibody (ab56234) at 1.25 µg/ml
Lane 1 : Molecular weight markers
Lane 2 : Jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 37 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
ab56234 at 4µg/ml, staining Human CEACAM6 in human lung tissue by immunohistochemistry on paraffin embedded tissue. The arrows indicate positively stained alveolar cells. Magnification 400X.
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