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Western blot - CEACAM6 antibody (ab56234)

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Immunohistochemistry (Paraffin-embedded sections) - CEACAM6 antibody (ab56234)

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Product Name
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CEACAM6 antibody
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See all CEACAM6 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to CEACAM6
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Immunogen
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A region within synthetic peptide: IQNPASANRS DPVTLNVLYG PDGPTISPSK ANYRPGENLN LSCHAASNPP, corresponding to internal sequence amino acids 217-266 of Human CEACAM6 BLAST 'IQNPASANRS DPVTLNVLYG PDGPTISPSK ANYRPGENLN LSCHAASNPP ' with or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions IHC-P: Use at a concentration of 4.0 - 8.0 µg/ml. WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 37 kDa).
ELISA titre using peptide based assay: 1/312500.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat cell lysate and human lung tissue.
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Cellular localization
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Cell Membrane. Lipid anchor, GPI anchor.
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Research areas
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Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Relevance
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The normal crossreacting antigen (NCA) or CEACAM6 belongs to the carcinoembryonic antigen (CEA) gene family. CEACAM6 is a GPI anchored membrane protein with a single N domain, which shows structural homology to the immunoglobulin variable domains, followed by two immunoglobulin constant like domains. Many breast, pancreatic, colonic and non small cell lung carcinoma lines express CEACAM6 and CEACAM5, and antibodies to both can affect tumor cell growth in vitro and in vivo. CEACAM6 expression is elevated in many solid tumors, but variable as a function of histotype. It may be a promising target for antibody based therapy.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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2.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CEACAM6 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CEACAM6 antibody images, references, products related to ab56234 and other tools.
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CEACAM6 antibody images:
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Western blot - CEACAM6 antibody (ab56234)
All lanes : CEACAM6 antibody (ab56234) at 1.25 µg/ml
Lane 1 : Molecular weight markers Lane 2 : Jurkat cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 37 kDa Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
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Immunohistochemistry (Paraffin-embedded sections) - CEACAM6 antibody (ab56234)
ab56234 at 4µg/ml, staining Human CEACAM6 in human lung tissue by immunohistochemistry on paraffin embedded tissue. The arrows indicate positively stained alveolar cells. Magnification 400X.
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Search PubMed (MEDLINE) for references to CEACAM6
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CEACAM6 antibody - more information
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Customer reviews (feedback) regarding CEACAM6 antibody |
Customer FAQs regarding CEACAM6 antibody |
Protocols for CEACAM6 antibody |
Price and availability of products related to CEACAM6 antibody |
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CEACAM6 antibody - related products:
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CEACAM6 antibody - other tools:
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Contact Abcam with a Technical Enquiry about CEACAM6 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"