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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-CEACAM6 antibody
See all CEACAM6 products (3) ...
Description
Rabbit polyclonal to CEACAM6
Tested applications
IHC-Fr, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
A region within synthetic peptide: IQNPASANRS DPVTLNVLYG PDGPTISPSK ANYRPGENLN LSCHAASNPP, corresponding to internal sequence amino acids 217-266 of Human CEACAM6
IQNPASANRS DPVTLNVLYG PDGPTISPSK ANYRPGENLN LSCHAASNPP
Positive control
Jurkat cell lysate and human lung tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab56234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr
One star (1 Abreview)- 1 Image
WB
- 1 Image
IHC-P
Application notes
IHC-P: Use at a concentration of 4.0 - 8.0 µg/ml.
IHC-Fr: Use at an assay dependent dilution (PMID 20220088).
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 37 kDa).
ELISA titre using peptide based assay: 1/312500.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Sequence similarities
Belongs to the immunoglobulin superfamily. CEA family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP40199
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Carcinoembryonic antigen related cell adhesion molecule 6 antibody
- Carcinoembryonic antigen-related cell adhesion molecule 6 antibody
- CD 66c antibody
see all
Database links
- Entrez Gene: 4680 Human
- Omim: 163980 Human
- SwissProt: P40199 Human
- Unigene: 466814 Human
Anti-CEACAM6 antibody images:
Western blot - CEACAM6 antibody (ab56234)
All lanes : Anti-CEACAM6 antibody (ab56234) at 1.25 µg/ml
Lane 1 : Molecular weight markers
Lane 2 : Jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 37 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
Immunohistochemistry (Paraffin-embedded sections) - CEACAM6 antibody (ab56234)
ab56234 at 4µg/ml, staining Human CEACAM6 in human lung tissue by immunohistochemistry on paraffin embedded tissue. The arrows indicate positively stained alveolar cells. Magnification 400X.
References for Anti-CEACAM6 antibody (ab56234)
This product has been referenced in:
- Allen CEet al. Cell-specific gene expression in Langerhans cell histiocytosis lesions reveals a distinct profile compared with epidermal Langerhans cells. J Immunol 184:4557-67 (2010). IHC-Fr; Human.Read more (PubMed: 20220088) »
See 1 publication for this product
Publishing research using ab56234? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"