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Read our guarantee »Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Anti-CEACAM6 antibody
See all CEACAM6 products (3) ...
Rabbit polyclonal to CEACAM6
IHC-Fr, WB, IHC-Pmore details
Reacts with
Human
A region within synthetic peptide: IQNPASANRS DPVTLNVLYG PDGPTISPSK ANYRPGENLN LSCHAASNPP, corresponding to internal sequence amino acids 217-266 of Human CEACAM6
IQNPASANRS DPVTLNVLYG PDGPTISPSK ANYRPGENLN LSCHAASNPP
Jurkat cell lysate and human lung tissue.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
Polyclonal
IgG
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Our Abpromise guarantee covers the use of ab56234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at a concentration of 4.0 - 8.0 µg/ml.
IHC-Fr: Use at an assay dependent dilution (PMID 20220088).
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 37 kDa).
ELISA titre using peptide based assay: 1/312500.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Belongs to the immunoglobulin superfamily. CEA family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cell membrane.
Target information above from: UniProt accessionP40199
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - CEACAM6 antibody (ab56234)

All lanes : Anti-CEACAM6 antibody (ab56234) at 1.25 µg/ml
Lane 1 : Molecular weight markers
Lane 2 : Jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 37 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
Immunohistochemistry (Paraffin-embedded sections) - CEACAM6 antibody (ab56234)

ab56234 at 4µg/ml, staining Human CEACAM6 in human lung tissue by immunohistochemistry on paraffin embedded tissue. The arrows indicate positively stained alveolar cells. Magnification 400X.
This product has been referenced in:
See 1 publication for this product
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Concentration of lot no. is
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All lanes : Anti-CEACAM6 antibody (ab56234) at 1.25 µg/ml
Lane 1 : Molecular weight markers
Lane 2 : Jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 37 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%

ab56234 at 4µg/ml, staining Human CEACAM6 in human lung tissue by immunohistochemistry on paraffin embedded tissue. The arrows indicate positively stained alveolar cells. Magnification 400X.
2
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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