Recombinant Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP709Y] to CEBP Alpha/CEBPA
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-CEBP Alpha/CEBPA antibody [EP709Y]
See all CEBP Alpha/CEBPA primary antibodies -
Description
Rabbit monoclonal [EP709Y] to CEBP Alpha/CEBPA -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- U937 cell lysate
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP709Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
|
Notes |
---|
WB
1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
Target
-
Function
C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. -
Sequence similarities
Belongs to the bZIP family. C/EBP subfamily.
Contains 1 bZIP domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 1050 Human
- Entrez Gene: 12606 Mouse
- Entrez Gene: 24252 Rat
- Omim: 116897 Human
- SwissProt: P49715 Human
- SwissProt: P53566 Mouse
- SwissProt: P05554 Rat
- Unigene: 76171 Human
see all -
Alternative names
- Apoptotic cysteine protease antibody
- Apoptotic protease Mch 5 antibody
- C/EBP alpha antibody
see all
Images
-
All lanes : Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764) at 1/1000 dilution (Purified)
Lane 1 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
Lane 2 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Rat liver tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDaThis antibody detects high backgrounds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (51)
ab40764 has been referenced in 51 publications.
- Zhang Q et al. Effects of 4-nonylphenol on adipogenesis in 3T3-L1 preadipocytes and C3H/10T1/2 mesenchymal stem cells. J Appl Toxicol 42:588-599 (2022). PubMed: 34553387
- Li J et al. Purinergic 2X7 receptor is involved in adipogenesis and lipid degradation. Exp Ther Med 23:81 (2022). PubMed: 34934450
- Wang Y et al. AdipoRon exerts opposing effects on insulin sensitivity via fibroblast growth factor 21-mediated time-dependent mechanisms. J Biol Chem 298:101641 (2022). PubMed: 35090894
- Liang X et al. Angiotensin II Inhibits Adipogenic Differentiation and Promotes Mature Adipocyte Browning through the Corepressor CtBP1. Biomedicines 10:N/A (2022). PubMed: 36551887
- Wang L et al. miR-34a Regulates Lipid Droplet Deposition in 3T3-L1 and C2C12 Cells by Targeting LEF1. Cells 12:N/A (2022). PubMed: 36611960