![Western blot - CEBP Alpha antibody [EP709Y] (ab40764) image](#ab40764.bmp)
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Product name
Anti-CEBP Alpha antibody [EP709Y]
See all CEBP Alpha products (13) ...
Description
Rabbit monoclonal [EP709Y] to CEBP Alpha
Tested applications
EMSA, WB, Flow Cyt, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to residues in the C-term of human CEBP Alpha
Positive control
U937 cell lysate
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP709Y
Isotype
IgG
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Western blot - CEBP Alpha antibody [EP709Y] (ab40764)
![Western blot - CEBP Alpha antibody [EP709Y] (ab40764) image](/ps/datasheet/Images/40/ab40764/ab40764.bmp)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
EMSA: Use at an assay dependent concentration. PubMed: 22184113
WB: 1/5000 - 1/20000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
Flow Cyt
Flow Cyt: 1/30.Flow Cyt: 1/30.
IP
IP: 1/50.IP: 1/50.
Application notes
Is unsuitable for ,ICC/IF or IHC.
Target
Function
C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
Sequence similarities
Belongs to the bZIP family. C/EBP subfamily.
Contains 1 bZIP domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP49715
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apoptotic cysteine protease antibody
- Apoptotic protease Mch 5 antibody
- C/EBP alpha antibody
see all
Database links
- Entrez Gene: 1050 Human
- Entrez Gene: 12606 Mouse
- Entrez Gene: 24252 Rat
- Omim: 116897 Human
- SwissProt: P49715 Human
- SwissProt: P53566 Mouse
- SwissProt: P05554 Rat
- Unigene: 76171 Human
see all
Anti-CEBP Alpha antibody [EP709Y] images:
Western blot - CEBP Alpha antibody [EP709Y] (ab40764)
Anti-CEBP Alpha antibody [EP709Y] (ab40764) at 1/20000 dilution + U937 cell lysate at 10 µg
Predicted band size : 43 kDa
Observed band size : 43 kDa
References for Anti-CEBP Alpha antibody [EP709Y] (ab40764)
This product has been referenced in:
- Marzook H et al. Metastasis-associated protein 1 drives tumor cell migration and invasion through transcriptional repression of RING finger protein 144A. J Biol Chem 287:5615-26 (2012). WB, EMSA, IP; Human.Read more (PubMed: 22184113) »
- Kilroy G et al. The ubiquitin ligase Siah2 regulates PPAR? activity in adipocytes. Endocrinology 153:1206-18 (2012).Read more (PubMed: 22294748) »
See all 2 publications for this product
Publishing research using ab40764? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"