Anti-CEBP Alpha antibody [EP709Y] (ab40764)

Overview

• Product nameAnti-CEBP Alpha antibody [EP709Y]See all CEBP Alpha primary antibodies ...
• Description
  Rabbit monoclonal [EP709Y] to CEBP Alpha
• Tested applicationsEMSA, WB, Flow Cyt, IP more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide corresponding to residues in the C-term of human CEBP Alpha

• Positive controlU937 cell lysate
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEP709Y
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Fatty acid oxidation

  • Cancer
  • Signal transduction
  • Other

  • Cardiovascular
  • Lipids / Lipoproteins
  • Fatty Acids
  • Synthesis

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Transcription Factors

Applications

Target

• FunctionC/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
• Sequence similaritiesBelongs to the bZIP family. C/EBP subfamily.
  Contains 1 bZIP domain.
• Cellular localizationNucleus.

Target information above from: UniProt accession P49715 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1050 Human
  • Entrez Gene: 12606 Mouse
  • Entrez Gene: 24252 Rat
  • Omim: 116897 Human
  • SwissProt: P49715 Human
  • SwissProt: P53566 Mouse
  • SwissProt: P05554 Rat
  • Unigene: 76171 Human

  • Unigene: 349667 Mouse
  • Unigene: 153603 Rat
  • Unigene: 204833 Rat

  see all

• Alternative names
  Apoptotic cysteine protease antibodyApoptotic protease Mch 5 antibodyC/EBP alpha antibody

  C/ebpalpha antibodyCAP4 antibodyCaspase 8 precursor antibodyCBF-A antibodyCCAAT Enhancer Binding Protein alpha antibodyCCAAT/enhancer binding protein (C/EBP), alpha antibodyCCAAT/enhancer-binding protein alpha antibodyCEBP antibodyCEBP A antibodyCEBP alpha antibodyCebpa antibodyCEBPA_HUMAN antibodyFADD homologous ICE/CED 3 like protease antibodyFADD like ICE antibodyFLICE antibodyICE like apoptotic protease 5 antibodyICE8 antibodyMACH antibodyMCH5 antibodyMORT1 associated CED 3 homolog antibody

  see all

Anti-CEBP Alpha antibody [EP709Y] images

• 

  Western blot - CEBP Alpha antibody [EP709Y] (ab40764)
  Anti-CEBP Alpha antibody [EP709Y] (ab40764) at 1/20000 dilution + U937 cell lysate at 10 µg
  
  Predicted band size : 43 kDa
  Observed band size : 43 kDa
  
• 

  Flow Cytometry-Anti-CEBP Alpha antibody [EP709Y](ab40764)
  Overlay histogram showing HeLa cells stained with ab40764 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40764, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.