Overview

  • Product nameAnti-CEBP Alpha antibody [EP709Y]See all CEBP Alpha primary antibodies ...
  • Description
    Rabbit monoclonal [EP709Y] to CEBP Alpha
  • Tested applicationsEMSA, WB, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the C-term of human CEBP Alpha

  • Positive control
    • U937 cell lysate
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab40764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
EMSA Use at an assay dependent concentration. PubMed: 22184113
WB 1/500. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
Flow Cyt 1/30.
IP Use at an assay dependent concentration.
  • Application notesIs unsuitable for ICC/IF or IHC.
  • Target

    • FunctionC/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
    • Sequence similaritiesBelongs to the bZIP family. C/EBP subfamily.
      Contains 1 bZIP domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Apoptotic cysteine protease antibody
      • Apoptotic protease Mch 5 antibody
      • C/EBP alpha antibody
      • C/ebpalpha antibody
      • CAP4 antibody
      • Caspase 8 precursor antibody
      • CBF-A antibody
      • CCAAT Enhancer Binding Protein alpha antibody
      • CCAAT/enhancer binding protein (C/EBP), alpha antibody
      • CCAAT/enhancer-binding protein alpha antibody
      • CEBP antibody
      • CEBP A antibody
      • CEBP alpha antibody
      • Cebpa antibody
      • CEBPA_HUMAN antibody
      • FADD homologous ICE/CED 3 like protease antibody
      • FADD like ICE antibody
      • FLICE antibody
      • ICE like apoptotic protease 5 antibody
      • ICE8 antibody
      • MACH antibody
      • MCH5 antibody
      • MORT1 associated CED 3 homolog antibody
      see all

    Anti-CEBP Alpha antibody [EP709Y] images

    • Anti-CEBP Alpha antibody [EP709Y] (ab40764) at 1/20000 dilution + U937 cell lysate at 10 µg

      Predicted band size : 43 kDa
      Observed band size : 43 kDa
    • Overlay histogram showing HeLa cells stained with ab40764 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40764, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    References for Anti-CEBP Alpha antibody [EP709Y] (ab40764)

    This product has been referenced in:
    • Marzook H  et al. Metastasis-associated protein 1 drives tumor cell migration and invasion through transcriptional repression of RING finger protein 144A. J Biol Chem 287:5615-26 (2012). WB, EMSA, IP ; Human . Read more (PubMed: 22184113) »
    • Kilroy G  et al. The ubiquitin ligase Siah2 regulates PPAR? activity in adipocytes. Endocrinology 153:1206-18 (2012). Read more (PubMed: 22294748) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"