![Western blot - CEBPB antibody [E299] (ab32358) image](#ab32358_1.jpg)
![Immunocytochemistry/ Immunofluorescence - CEBPB antibody [E299] (ab32358) image](#ab32358_2.jpg)
![Western blot - CEBP Beta antibody [E299] (ab32358) image](#CEBP-Beta-Primary-antibodies-ab32358-4.jpg)
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Product name
Anti-CEBP Beta antibody [E299]
See all CEBP Beta products (10) ...
Description
Rabbit monoclonal [E299] to CEBP Beta
Specificity
This antibody is specific for the three CEBPB isoforms (LAP*, LAP and LIP).
Tested applications
EMSA, ICC, WB, ICC/IF, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to residues in the C terminus of Rat CEBPB.
Positive control
WB: PC12 cell lysate. IF: Hela cells.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
E299
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
EMSA
- 1 Image
ICC/IF
IP
Application notes
EMSA: Use at an assay dependent dilution (PMID 18462018).
ICC/IF: 1/50 - 1/100.
IP: 1/20.
WB: 1/1000. Predicted molecular weight: 36 kDa.
Is unsuitable for Flow Cyt or IHC.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation.
Tissue specificity
Expressed at low levels in the lung, kidney and spleen.
Sequence similarities
Belongs to the bZIP family. C/EBP subfamily.
Contains 1 bZIP domain.
Domain
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
Post-translational
modifications
Sumoylated by polymeric chains of SUMO2 or SUMO3.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP17676
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AGP/EBP antibody
- C EBP beta antibody
- C/EBP beta antibody
see all
Database links
- Entrez Gene: 1051 Human
- Entrez Gene: 12608 Mouse
- Entrez Gene: 24253 Rat
- Omim: 189965 Human
- SwissProt: P17676 Human
- SwissProt: P28033 Mouse
- SwissProt: P21272 Rat
- Unigene: 517106 Human
see all
Anti-CEBP Beta antibody [E299] images:
Western blot - CEBPB antibody [E299] (ab32358)
![Western blot - CEBPB antibody [E299] (ab32358)](/ps/datasheet/Images/32/ab32358/ab32358_1.jpg)
Anti-CEBP Beta antibody [E299] (ab32358) at 1/1000 dilution + PC12 cell lysate.
Predicted band size : 36 kDa
Observed bands
LAP*: 38kDa
LAP: 35kDa
LIP: 20kDa
Immunocytochemistry/ Immunofluorescence - CEBPB antibody [E299] (ab32358)
![Immunocytochemistry/ Immunofluorescence - CEBPB antibody [E299] (ab32358)](/ps/datasheet/Images/32/ab32358/ab32358_2.jpg)
Ab32358, at a 1/50 dilution, staining CEBPB in Hela cells by Immunofluorescence.
Western blot - CEBP Beta antibody [E299] (ab32358)
![Western blot - CEBP Beta antibody [E299] (ab32358)](/ps/datasheet/images/32/ab32358/CEBP-Beta-Primary-antibodies-ab32358-4.jpg)
All lanes : Anti-CEBP Beta antibody [E299] (ab32358) at 1/500 dilution
Lane 1 : 3T3 Cells
Lane 2 : MCF7 Cells
Lane 3 : PC12 Cells
Lane 4 : Rat Spleen Lysate
Lane 5 : Rat Kidney Lysate
Lane 6 : Rat Heart Lysate
Lane 7 : Rat Brain Lysate
Lane 8 : Mouse Spleen Lysate
Lane 9 : Mouse Kidney Lysate
Lane 10 : Mouse Heart Lysate
Lane 11 : Mouse Brain Lysate
Predicted band size : 36 kDa
References for Anti-CEBP Beta antibody [E299] (ab32358)
This product has been referenced in:
- Michas G et al. Reciprocal regulation of 11ß-hydroxysteroid dehydrogenase 1 and glucocorticoid receptor expression by dexamethasone inhibits human coronary artery smooth muscle cell proliferation in vitro. Mol Cell Biochem 346:69-79 (2011). WB; Human.Read more (PubMed: 20922465) »
- Pal R et al. C/EBPbeta regulates transcription factors critical for proliferation and survival of multiple myeloma cells. Blood 114:3890-8 (2009). WB, IHC-P; Human.Read more (PubMed: 19717648) »
See all 3 publications for this product
Publishing research using ab32358? Please let us know so that we can cite the reference in this datasheet
![CEBP Beta antibody [E299] for WB in Human (32358)](/ps/datasheet/images/32/ab32358/CEBP-Beta-Primary-antibodies-ab32358-3.jpg)
![Anti-CEBP Beta antibody [E299] for Western blot in Human (32358)](/ps/datasheet/images/32/ab32358/CEBP-Beta-Primary-antibodies-ab32358-12.jpg)
![CEBP Beta antibody [E299] for ICC in Human (32358)](/ps/datasheet/images/32/ab32358/CEBP-Beta-Primary-antibodies-ab32358-1.png)
![CEBP Beta antibody [E299] for ICC in Mouse (32358)](/ps/datasheet/images/32/ab32358/CEBP-Beta-Primary-antibodies-ab32358-2.png)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"