Overview

  • Product name
  • Description
    Rabbit polyclonal to CEBPE
  • Specificity
    This antibody detects endogenous levels of total CEBPE protein.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthesized non-phosphopeptide derived from human CEBPE around the phosphorylation site of threonine 74.

  • Positive control
    • Human brain tissue and extracts from HuvEc cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab59384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

Target

  • Function
    C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
  • Tissue specificity
    Strongest expression occurs in promyelocyte and late-myeloblast-like cell lines.
  • Sequence similarities
    Belongs to the bZIP family. C/EBP subfamily.
    Contains 1 bZIP domain.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C/EBP epsilon antibody
    • CCAAT/enhancer binding protein (C/EBP) epsilon antibody
    • CCAAT/enhancer binding protein epsilon antibody
    • CCAAT/enhancer-binding protein epsilon antibody
    • CEBPE antibody
    • CEBPE_HUMAN antibody
    • CRP 1 antibody
    • CRP1 antibody
    see all

Images

  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab59384 at a 1/50 dilution.

    Left image un-treated.

    Right image treated with peptide.

  • All lanes : Anti-CEBPE antibody (ab59384) at 1/500 dilution

    Lane 1 : Extracts from HuvEc cells, treated with Insulin (0.01U /ml, 15mins).
    Lane 2 : Extracts from HuvEc cells, treated with Insulin (0.01U /ml, 15mins) plus peptide.


    Predicted band size : 31 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

References

ab59384 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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