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Products:Epigenetics and Nuclear Signaling >> Histones >> Variants
Overview
Product name
Anti-CENPA antibody - ChIP Grade
See all CENPA products (10) ...
Description
Rabbit polyclonal to CENPA - ChIP Grade
Tested applications
ICC/IF, WB, ChIPmore details
Cross reactivity
Reacts with
Mouse
Predicted to work with
Chinese Hamster
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Mouse CENPA.
(Peptide available as ab33564.)
Positive control
NIH 3T3 Whole cell lysate (Mouse embryonic fibroblast cell line); MEF1.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Cell cycle >> Chromosome Structure >> Centromere
Epigenetics and Nuclear Signaling >> Chromosome Structure >> Centromeres
Epigenetics and Nuclear Signaling >> Histones >> Variants
Applications
Show applications key
Our Abpromise guarantee covers the use of ab33565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ChIP
Application notes
RNA NChIP: Use at an assay dependent dilution. (Pubmed 19542185)
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.
Sequence similarities
Belongs to the histone H3 family.
Domain
The CATD (CENPA targeting domain) region is responsible for the more compact structure of nucleosomes containing CENPA and is necessary and sufficient to mediate the localization into centromeres.
Post-translational
modifications
Ubiquitinated (Probable). Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway.
Phosphorylation of Ser-7 by Aurora-A/STK6 and Aurora-B/STK12 during prophase is required for localization of Aurora-A/STK6 and Aurora-B/STK12 at inner centromere and is essential for kinetochore function. Initial phosphorylation during prophase is mediated by Aurora-A/STK6 and is maintained by Aurora-B/STK12.
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height.
Target information above from: UniProt accessionP49450
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CENP A antibody
- CENP-A antibody
- cenpa antibody
see all
Database links
- Entrez Gene: 12615 Mouse
- SwissProt: O35216 Mouse
- Unigene: 290563 Mouse
Anti-CENPA antibody - ChIP Grade images:
Western blot - CENPA antibody (ab33565)
Lane 1 : Marker
Lanes 2 - 3 : Anti-CENPA antibody - ChIP Grade (ab33565) at 1 µg/ml
Lane 1 : As above
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179) at 20 µg
Lane 3 : MEF1 at 20 µg
Secondary
Lanes 2 - 3 : IRDye 680 conjugated Goat anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 16 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - CENPA antibody (ab33565)
ICC/IF image of ab33565 stained MEF1 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab33565, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-CENPA antibody - ChIP Grade (ab33565)
This product has been referenced in:
- Ferri Fet al. Non-coding murine centromeric transcripts associate with and potentiate Aurora B kinase. Nucleic Acids Res 37:5071-80 (2009). WB, ChIP; Mouse.Read more (PubMed: 19542185) »
See 1 publication for this product
Publishing research using ab33565? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"