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Products:Epigenetics and Nuclear Signaling >> Histones >> Variants
Overview
Product name
Anti-CENPA antibody
See all CENPA products (10) ...
Description
Rabbit polyclonal to CENPA
Specificity
Detects endogenous levels of total CENPA protein.
Tested applications
ELISA, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic non-phosphopeptide derived from human CENPA around the phosphorylation site of serine 7 (R-R-SP-R-K).
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
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Immunocytochemistry/ Immunofluorescence - CENPA antibody (ab62688)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab62688 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/10000ELISA: 1/10000
ICC/IF: 1/500 - 1/1000.
Target
Function
Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.
Sequence similarities
Belongs to the histone H3 family.
Domain
The CATD (CENPA targeting domain) region is responsible for the more compact structure of nucleosomes containing CENPA and is necessary and sufficient to mediate the localization into centromeres.
Post-translational
modifications
Ubiquitinated (Probable). Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway.
Phosphorylation of Ser-7 by Aurora-A/STK6 and Aurora-B/STK12 during prophase is required for localization of Aurora-A/STK6 and Aurora-B/STK12 at inner centromere and is essential for kinetochore function. Initial phosphorylation during prophase is mediated by Aurora-A/STK6 and is maintained by Aurora-B/STK12.
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height.
Target information above from: UniProt accessionP49450
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CENP A antibody
- CENP-A antibody
- cenpa antibody
see all
Database links
- Entrez Gene: 1058 Human
- Omim: 117139 Human
- SwissProt: P49450 Human
- Unigene: 1594 Human
Anti-CENPA antibody images:
Immunocytochemistry/ Immunofluorescence - CENPA antibody (ab62688)
ab62688 at 1/500 - 1/1000 dilution staining CENPA in HeLa cells (treated with Forskolin (40nM, 30mins) or untreated) by Immunofluorescence,
References for Anti-CENPA antibody (ab62688)
ab62688 has not yet been referenced specifically in any publications.
Publishing research using ab62688? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"