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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab4488? Please let us know so that we can cite the reference in this datasheet
ab4488 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot
All lanes : Anti-CENPA (di methyl K9) antibody (ab4488) at 1/500 dilution
Lane 1 : HeLa Cell Lysate at 20 µg
Lane 2 : HeLa nuclear lysate at 20 µg
Lane 3 : Calf thymus histone prep at 1 µg
Lane 4 : HeLa Cell Lysate at 20 µg with
Lane 5 : HeLa nuclear lysate at 20 µg with
Lane 6 : Calf thymus histone prep at 1 µg with
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 16 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
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