Anti-CENPB antibody (ab167361)
Key features and details
- Mouse polyclonal to CENPB
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CENPB antibody
See all CENPB primary antibodies -
Description
Mouse polyclonal to CENPB -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human CENPB aa 1-600. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Recombinant Human CENPB protein (ab73636) can be used as a positive control in WB. A431 cell lysate, CENPB transfected 293T cell line lysate, HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab167361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes. -
Sequence similarities
Contains 1 HTH CENPB-type DNA-binding domain.
Contains 1 HTH psq-type DNA-binding domain. -
Cellular localization
Nucleus. Chromosome > centromere. - Information by UniProt
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Database links
- Entrez Gene: 1059 Human
- Omim: 117140 Human
- SwissProt: P07199 Human
- Unigene: 516855 Human
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Alternative names
- CENP B antibody
- CENP-B antibody
- CENPB antibody
see all
Images
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Anti-CENPB antibody (ab167361) at 1 µg/ml + A431 cell lysate at 50 µg
Secondary
Goat anti-Mouse IgG HRP at 1/2500 dilution
Predicted band size: 65 kDa -
All lanes : Anti-CENPB antibody (ab167361) at 1 µg/ml
Lane 1 : CENPB transfected 293T cell line lysate
Lane 2 : Non-transfected 293T cell line lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat anti-Mouse IgG HRP at 1/2500 dilution
Predicted band size: 65 kDa -
Immunofluorescence analysis of HeLa cells labeling CENPB using ab167361 at 10 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab167361 has been referenced in 2 publications.
- Murillo-Pineda M et al. Induction of spontaneous human neocentromere formation and long-term maturation. J Cell Biol 220:N/A (2021). PubMed: 33443568
- Mitra S et al. Genetic screening identifies a SUMO protease dynamically maintaining centromeric chromatin. Nat Commun 11:501 (2020). PubMed: 31980633