For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome.
Recombinant full length protein (Human).
Our Abpromise guarantee covers the use of ab5093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|IP||Use at µg/mg of lysate. Use at a concentration of 3 µg/ml per 300 µg of lysate.|
|ICC||Use at an assay dependent dilution.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 312 kDa. Only suitable for WB if IP is performed first.|
|Flow Cyt||Use 1µg for 106 cells.|
Kinetochores specific staining of HCT116 cells arrested in G2/M phase by nocodazole treatment. Methanol fixed cells were stained using mouse monoclonal [1H12] antibody to CENP-E ab5093 (green) and DAPI (blue).
This image was kindly supplied as part of the review submitted by Salvador Rodrigez-Nieto.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"