For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Recombinant full length protein (Human).
Our Abpromise guarantee covers the use of ab5093 in the following tested applications.
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration. Use at a concentration of 3 µg/ml per 300 µg of lysate.|
|ICC||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 312 kDa. Only suitable for WB if IP is performed first.|
|Flow Cyt||Use 1µg for 106 cells.|
Kinetochores specific staining of HCT116 cells arrested in G2/M phase by nocodazole treatment. Methanol fixed cells were stained using mouse monoclonal [1H12] antibody to CENP-E ab5093 (green) and DAPI (blue).
This image was kindly supplied as part of the review submitted by Salvador Rodrigez-Nieto.