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Synthetic peptide derived from within residues 2450 - 2550 of Human CENPE.
(Peptide available as ab24674.)
Our Abpromise guarantee covers the use of ab4163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution. PFA fixation has not given good results (see Abrevie by Kirk McManus). Methanol fixation seems more optimal.|
|WB||1/500. Detects a band of approximately 312 kDa (predicted molecular weight: 312 kDa).Can be blocked with Human CENPE peptide (ab24674).|
The band of 312kD (expected size) can be blocked by the immunising peptide (data not shown). It is possible that the lower band represents protein degradation in the extract.
ab4163 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"